Relative Study On The Expressive Significance Of Cyclooxygenase 2 And Nuclear Factor Kappa B With Cytotoxin-associated Gene A～+ Helicobactor Pylori In The Gastric Cancer And Precancerous Lesions

Objective: To investigate the expressive significance of cyclooxygenase 2 and nuclear factor kappa B with cytotoxin-associated gene A+ Helicobactor pylori in the gastric cancer and precancerous lesions and find the valuable experimental and theoretical proof for the prevention and treatment of gastric cancer.Methods: 121patients (37 with gastric cancer-GC,40 with intestinal metaplasia or dysplasia-IM+DYS, 44 with chronic superfial gastritis-CSG) biopsied under endoscopy for gastric mucosa were studied. ABC immunohistochemical methods were used to detect the expression of COX-2 and NF-KB in gastic mucosa. The CagA gene among the HP infected gastric mucosa was determined by PCR.Results: 1 In gastric mucosa lesion with HP infection CagA+ layer was 77.4%. The positive infectious rates of CagA+HP in CSG, IM+DYS, GC were 59.3 % 83.9 %, 85.5 %, respectively. The positive infectious rates of CagA+HP in IM+DYS and GC were significantly higher than that in CSG(P<0.05) ,there was no significantly difference in positive infectious rates of CagA+HP between IM+DYS and GC(P>0.05). 2 The positive expression rates of COX-2 in CSG, IM+DYS, GC were 45.1 %, 67.5%, 81.1 % ,respectively. The positive expression rates of NF-KB inCSG, IM+DYS, GC were 34.1 % 60%, 62.2%, respectively. Both of them in IM+DYS and GC were significantly higher than that in CSG(P<0.05). 3 Of all the patients with HP infection in gastic musoca lesion, the CagA+HP group had a significantly higher of the positive expression rates of COX-2 and NF- K B than that in the CagA-HP group(P<0.01). Comparing the CagA+HP group with the CagA-HP group, the former had a higher positive expression rate of COX-2 in CSG and GC than the latter(P<0.05),and had a higher positive expression rate of NF- K B in CSG and IM+DYS than the latter(P<0.05). 4 Of all the patients the expression intensity of COX-2 and NF-KB has plus relativity, r equal 0.476(P<0.05).Conclusions: The infection of CagA+HP plays an important role in the development of gastic cancer. Overexpression of COX-2 and NF-KB in patients with GC or precancerous lesions. The infection of CagA+HP can promote the expression rates of COX-2 and NF-KB in GC. The infection of CagA+HP, the promotion of expression of COX-2 and NF-KB may all play a role in the development/promotion of GC. Therefore, completely curing the HP infection and using NF- K B inhibitor and NS AIDs at the same time may be a effective method to prevent the development/promotion of GC.