| Laryngeal carcinoma is a commom malignant tumor in otolaryngology. Its morbidity was increased year by year. It has been the important topic of otolaryngology. At present assessment of patient's treatment and prognosis is dependent on pathologic grade, TNM classification and primary locus of LSCC. Recently many new tumor indicators were uninterruptly discovered and used, such as VEGF, PCNA, E-Cadherin and β-catenin. The relation with β - catenin and magliant tumor is a new investigation domain about maglinant behavior of tumor. The complex of E-cadherin-catenin is conformed by many transmembrane glycoproteins to responsible for calcium dependent intercellular adhesion. β - catenin is a multifunctional protein among them. First of all, it plays a important role in cell adhesion. β - catenin mutation or disfunction can lead to divergence of cell or invasion of tumor. Secondly, β-catenin is a downstream effector of the Wnt signaling pathway. The activation of Wnt signaling pathway can result in proliferation of cell and generation of tumor. The nearest investigation suggests that β-catenin plays a important role in the formation and progression of tumor and may be used as a independent prognostic indicator. However, study on the relationship with β-catenin and clinicopathological features, prognosis in LSCC is rare. It is necessary to study further. In this experiment, we detect expression of β-catenin by immunohistochemical method in 44 patients with LSCC to investigate the relation with β-catenin expression and clinicopathological chacteristics, prognosis. It may provide data for evaluating malignant behavior, prognosis and selecting treatment plan in LSCC.Materials and MethodsIn this study, 44 patients who were treated for laryngeal cancer were from August 1989 to September 2000 in the first affiliated hospital of medical college, Zhejiang University. All accepted no therapy for the tumor before their surgery and were proved to be squamous cell carconoma by pathology. All of patients were male. Their age ranged from 41 to 82, and the average age was 60.5. There were 22 glottic cancers, 21 supraglottic ones and 1 subglottic one. There were 17 high-differentiated cancers, 19 middle-differentiated ones and 8 low-differentiated ones. According to the TNM classification of UICC in 1997, Tl tumors were 4,and T2,T3,T4 tumors were 12,13,15 respectively. Tumors of I II III IV stage were 4,6,15,19 respectively. Among all 44 patients, there were 23 patients with cervical lymph node metastasis.In this experiment, we use immunohistochemical SP method to detect angiogenesis of LSCC tissues by β-catenin monoclonal antibody. The results were observed in light microscope. Membrane β-catenin expression was accounted by membrane β-catenin positive tumor cells/all counted tumor cells. Aberrant localization β-catenin expression was judged by occurrence of aberrant localization β-catenin positive cell. Both low membrane β-catenin expression and aberrant localization β-catenin expression are called as abnomal β - catenin expression.The data were analyzed by statistical package (SPSS 10.0 for windows). Main statistical methods were chi-square test, Kaplan-Meier survival.Results1.10 normal laryngeal tissue had nomal β-catenin expression. 44 laryngeal cancer tissue had 16 cases of normal β-catenin expression and 28 cases of abnomal β-catenin expression. There was a statistical difference of p-catenin expression between normal tissue and laryngeal cancer (P<0.01) .2. Membrane β-catenin expression revealed a reverse correlation with aberrant localization β - catenin expression (P <0.05) .3.High histological grading tumor had a significantly higher membrane p-catenin expression than middle and low histological grading tumor (P <0.05 ) . No correlation wasfound between membrane β-catenin expression and tumor T stage, cervical lymph node metastasis, clinical staging (P >0.05) .4. Aberrant localization P-catenin expression had no statistical significant relevance with clinical...
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