Effect Of Toll-Like Receptors' Pathway In Apoptosis Of Colon Cancer Cells Induced By Bifidobacteria Lipoteichoic Acid

Bifidobacterium lipoteichoic acid has the ability of inducing occurence of apoptosis in tumor cells in vitro and in vivo, but its mechanism is not clear. Toll-like receptors are a serial of receptors related to innate immunity and Toll-like receptor is the natural receptor of lipoteichoic acid. In the topic, we do experiments on Toll-like receptors in order to study the signal transduction pathway of bifidobacterium lipoteichoic acid in inducing apoptosis of colon cancer cells. After detecting the basal expression of TLRs, the matching receptor of LTA and alterating expression of it through RT-PCR, meanwhile detecting alteration of NF-κB protein expression, the continuous molecular of apoptosis pathway, through immunocytochemistry and western blot, we find that expression of TLR2 and TLR4 in mRNA level increases greatly. On the contrary, expression of NF-κB in protein level decrease deeply, at the same time, switch on the Caspase pathway to apoptosis. Therefore we establish the cascade reaction of Toll-like receptor and NF-κB in apoptosis signal transduction pathway of lipoteichoic acid on colon cancer cells, which provide the theoretical base and idea for mechanism of lipoteichoic acid`s anti-tumor effect, utility and development of bifidobacterium surface molecular.