Study On The Expression Of TLR2 And IL-1β Induced By Enterococci. Faecalis Lipoteichoic Acid On Human Periodontal Ligament Cells

Objective Enterococci. Faecalis is considered as one of the most important pathogens for refractory periapical disease. Lipoteichoic Acid is viewed as its main virulent factor. This study is aiming at investigating the expression of TLR2 and IL-1βof cultured human periodontal ligament cells (HPDLCs) activated by Enterococci. Faecalis Lipoteichoic Acid (E.faecalis LTA) so as to illustrate the effect of E.faecalis LTA on periapical disease.Methods (1) Human periodontal ligament cells were cultured and identified in vitro by explant method. (2) Flow cytometry was used to detect the expression of TLR2 on normal HPDLCs and infectious HPDLCs which were incubated with 0.1,1, 10μg/ml E.faecalis LTA for 24h, E.coli LPS was used as positive control. (3) IL-1βwas detected by ELISA after being incubated with LTA of the above concentration for 12, 24 and 48h or pretreated with 1:100 anti-TLR2 Ab for 1h and then co-cultured with 1μg/ml LTA for 24h, E.coli LPS was used as positive control.Results (1) Normal HPDLCs have little TLR2 expression. E.faecalis LTA could strengthen the expression. The difference has statistical significance(P<0.05). (2) IL-1βsecretion can be detected 12h after stimulation with LTA and increasingly escalated within 48h(P<0.05). (3) After pretreatment with anti-TLR2 antibody, the levels of IL-1βsecretion decreased(P>0.05).Conclusions (1) E.faecalis LTA could raise the expression of TLR2 on HPDLCs. (2) E.faecalis could add the production of IL-1βand the passible mechanism may be TLR2 pathway. (3) E.faecalis LTA may participate in the inflammatory process of periapical disease...