Experimental Study Of The Stimulative Effect Of Bupleurum And Citrus Extraction On Gastrointestinal Motility

ObjectiveTo compare the stimulative effect on gastrointestinal motility of water extraction ^ethanol extraction and ethanol water mixed extraction of Bupleurum and Citrus to screen out the optimum extraction (TOE) , to observe that it influenced on the gastrointestinal inhibitive model induced by Atropin and Morphine and the contents of nitric oxide (NO) in the gastric fundusN gastric antrum and jejunum tissue in order to explore the mechanism of the stimulative effect on gastrointestinal motility .Methods1. To select B. Scorzonerifolium Willd and Citrus aurantium to execute water extraction ,ethanol extraction and ethanol water mixed extraction respectively.2. Kunming mice were randomly divided into 11 groups,each group was 20 mice, negative control was distilled water group, positive control was Cisapride group, the rest were different dosage extraction groups, compared the stimulative effect of different dosage extractions on gastrointestinal motility with Blue Dxtran 2000 as a marker ,by relative pigment remainder rate inside stomach and average small intestinal propulsive ratio as guide lines.3. Kunming mice were randomly divided into 6 groups, each group was 15 mice ,they were distilled water group ,Atropin group ,Morphine group , water extraction of 3 times usual dosage (3WEOTUD) group , Morphine and 3WEOTUD group , Atropin and 3WEOTUD group respectively. To continue applying theabove the marker and guide lines to observe that 3WEOTUD influenced on the gastrointestinal inhibitive model induced by Atropin and Morphine.4. Wistar rats were randomly divided into 2 groups,one group was Chinese traditional medicine group, another was normal control group ,each group was 15 rats. The contents of nitric oxide (NO) in the gastric fundus^ gastric antrum and jejunum tissue were measured with the method of nitrate reductase at the same time ,the plasma motilin(MTL) level was detected with radioimmunoassay .5. Statistical analysis: All date were expressedas Mean SD and analyzed between two groups by T test with statistical software for SPSS 10.0 .Results1. Every extraction group except the ethanol extraction 14mg/g group compared with distilled water group, it was highly significant difference( P < 0. 01) or significant difference( P < 0. 05) , the water extraction of 3 times usual dosage compared with Cisapride group , it wasn't significant difference.2. To compared relative pigment remainder rate inside stomach and average small intestinal propulsive ratio respectively between two groups, B group comparing with A group ,D group comparing with A group ,D group comparing with E group ,E group comparing with F group and C group comparing with F Group were significant difference ( P < 0. 05) , but B group comparing with C group wasn' t significant difference( P > 0. 05 ).3. H group compared with G group on relative pigment remainder rate inside stomach and average small intestinal propulsive ratio, it was highly significant difference (P<0.01), H group compared with G group in plasma motilin, it was significant difference (P <0. 05) , the content of NO in the gastric fundus, gastric antrum and jejunum of H group compared with G group was highly significant difference( P < 0.01) .Conclusion1. 3WEOTUD was confirmed the optimum extraction .2. 3WEOTUD couldn't antagonize the gastrointestinal inhibitive effect induced by Atropin , but it could antagonize gastrointestinal inhibiting effect induced by Morphine.3.3WEOTUD could promote gastrointestinal motility of rats obviously and enhance plasma motilin level ,the content of NO in the gastric fundus tissue of rats was increased significantly , but the content of NO in the gastric antrum and jejunum tissue were decreased significantly.