The Experimental Study Of Renal Capsular Block On Bidirectional Regulation Of Gastrointestinal Motility

Objective:Taking the rats as the research object which have abnormal gastrointestinal motility and observing the levers of gastric residual rate, intestine propulsion rate,brain-gut peptide hormones and enteric neurotransmitters in order to explore the mechanism of renal capsular block on the regulation of gastrointestinal motility.Methods:1. seventy-two Sprague-Dawley rats were randomly divided into three groups. Group A was non-model group, group B was the inhibition model produced by intraperitoneal injection of atropine,group C was the hyperactivity model produced by intraperitoneal injection of neostigmine. Each group was divided into three subgroups, group A1,group B1 and group C1 were the sham operated group, which exposed the kidney and renal capsule; group A2,group B2 and group C2 were the renal capsule injected NS groups;group A3,group B3 and group C3 were the renal capsule injected lidocaine groups2.We fed the rats with semi-solid food which contains activated carbon. Calculating the rate of gastric residual by weighing the whole weight and net weight of the stomach and measuring the rate of intestinal propulsion with activated carbon to test the dynamical variety of rat stomach and intestine.3.Radioimmunoassay was used to detect the level of motilin(MTL) in tissues of gastric antrum and hypothalamus in order to observe the effect of renal capsular block on brain-gut peptides.4.Nitrate reductase method was used to detect the level of nitric oxide (NO) in colon tissues in order to observe the effect of renal capsular block on brain-gut peptides.Results:1.The lever of gastric residual rate in group A1 was more lower than that in group B1,while higher than that in group C1 (p< 0.05). The levers of intestine propulsion rate and motilin in group A1 were more higher than that in group B1,while lower than that in group C1 (p< 0.05).2.There was no significant difference in the levers of gastric residual rate,intestine propulsion rate and motilin between the sham operated group and the renal capsule injected NS group (p> 0.05).3.The lever of gastric residual rate in group A1 was more higher than that in group A3 and the levers of intestine propulsion rate and motilin in tissues of hypothalamus in group A1 were more lower than that in group A3 (p< 0.05). There was no significant difference in the levers of motilin in tissues of gastric antrum between group A1 and group A3 (p> 0.05). There was no significant difference in the levers of gastric residual rate and motilin between group A2 and group A3(p> 0.05). The lever of intestine propulsion rate in group A2 was lower than group A3. The lever of gastric residual rate in group B1 and group B2 was more higher than that in group B3 and the levers of intestine propulsion rate and motilin in group B1 and group B2 were more lower than that in group B3(p<0.05). The lever of gastric residual rate in group C1 and group C2 was more lower than that in group C3 and the levers of intestine propulsion rate and motilin in group C1 and group C2 were more higher than that in group C3 (p< 0.05).4.There was no significant difference in the lever of NO among the groups(p>0.05).Conclution:1.Renal capsule block can speed up the lower gastrointestinal motility caused by atropine and slow down the higher gastrointestinal motility caused by neostigmine. This shows that renal capsular block can bidirectionally regulate gastrointestinal motility.2.Renal capsule block has no effect on the lever of NO with normal nitrergic nerves.