| OBJECTIVE To study the effects of exogenous inosine on expressions of growth- associated protein-43 gene (GAP-43 mRNA), Nogo protein gene (Nogo-A mRNA) and neurological grade after focal cerebral ischemia in rats, and to ensure the possible of inosine to treat central nervous system injury. METHODS 60 adult female rats were divided randomly into inosine-treated group and controlled group. The rats were received left middle cerebral artery occlusion of 1.5h and different hours of reperfrsion. Neurological grade evaluation and in situ hybridization were used to examinate the behaviorcal recovery and expressions of GAP-43 mRNA and Nogo-A mRNA on the same rats subjected to 2h,12h, 1d, 2d, 3d, 7d, 14d of reperfusion and sham-operated group ( n=4 ).RESULTS (1)There was improvement in the score of neurologic grade in inosine-treated group compared with controlled group at reperfusion of 7d and 14d. (2)GAP-43 mRNA expression in ischemia cortex and striatum was not remarkable in sham-operated group. The expression increased markedly after inosine treatment at reperfusion of 12h,7d compared with controlled group. (3)Nogo-A mRNA expression in ischemia cortex and striatum was not remarkable in sham-operated group. The expression increased markedly after inosine treatment at reperfusion of 12h, 2d, 3d and decreased significantly at reperfusion of 7d compared with controlled group. (p<0. 05) CONCLUSION It is suggested that inosine can improve behavioral outcome of MCAO rats . The enhanced neurological function might be partially due to the upregulation of GAP-43 mRNA and the alterment of Nogo-A mRNA which are correlated with neuronal regeneration.
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