| Chronic renal failure (CRE)is based on all kinds of kidney diseases, slowly appearances the lower renal function until to failure .The CRF patients are always accompanied with many kinds of complications, such as anemia, disfunction of cardiovascar system, disfunction of gastrointestinal tract, thrombose, etc. These patients are also accompanied with malnutrition and lower immunity which cause an unpleased living quality and short-living life. In these complications anemia is perhaps one of the most important features that effect CRF patients. EPO as an endocrine hormone the satisfied effect to correct anemia has been known of us ,which can increase erythropoietin and elevate hematocrit, and has been used extensively from the r-HuEPO was gained by Powell who used DAN recombination technology in 1985. Now, the study of EPO is not confined in increasing the number of RBC, the other more and more physiological functions has been found, such as to protect neuron ,promote intestinal mucosa development ,to increase the gonadotropin releasing.,and to increase the susceptibility to radiopraxis, etc. Nutrition deficiency ,lower immunity and high coagulation state are the main features in CRF patients. Because of the failing of renal function, CRF patients can not ingest and absorb food efficiency in a long time ,then lead to malnutrition. Accumulation of toxin ,anemia, and increasing of immunocompound lead to the lower function of RBC immunity and lower function of body immunity in CRF patients, and thus ,make them easy to get inflammation ,which will worsen malnutrition then. The large number of immunocompound in circulation leads to the action of many aterials such as complement and platelet, these materials cause the high coagulation state in CRF patients .Therefor, anemia, malnutrition ,increased coagulant materials and lower RBC immunity are the correlate factors react each other in CRF patients. The object of this paper is to observe the concentration change of PA, TRF, RBP, FDP in blood, and the change of RBC-C3bRR and RBC-ICRR in the group with or without r-HuEPO injection ,and calculate the correlation between them and HB or CRE concentration .Method: the study comprised two groups of 45 matched cases ,CRF patients with r-HuEPO injection (25cases),CRF patients without r-HuEPO injection (20cases). Took the all blood samples in the morning. In order to reduce the effect of hemodialysis to the study ,we took the sample in the morning before the next hemodialysis for the patients with r-HuEPO injection. All these patients used the r-HuEPO more than one month. According to the theory of the C3b receptor on the RBC membrane can adhere to saccharomycete sensitized by complement and form a round , and the theory of C3b molecule of IC or antigen-complement compound on RBC membrane can adhere to unsensitized saccharomycete and form a round, we washed the anticoagulate blood sample by normal saline ,made up to 1.25×107/ml RBC solution. Then after mixing the sensitized saccharomycete or unsensitized saccharomycete with these solution, water bathed 30 minutes, fixed ,colored ,counted the round rate with microscope (one RBC was adhered with more than two saccharomycetes is a round) .Besides, according to the ELISA method we added anti-human PA ,RPB ,TRF ,FDP ntibody to enzyme-labeled-antibody, color developed ,and measured the OD value at 490nm, and then calculated the concentration of PA, RBP, TRF, FDP .Rusults :1. HB, PA, RBP, TRF concentration are significantly higher than that in the guoup without r-HuEPO injection (P<0.05). 2. FDP concentration is significantly lower than that in the guoup without r-HuEPO injection (P <0.05). 3. RBC-C3bRR is significantly higher than that in the group without r-HuEPO injection (P<0.05), and RBC-ICRR is significantly lower than that in the group without r-HuEPO injection (P<0.05). 4. The change of CRE concentration is not obvious in the two guoups . 5. There is a positive correlation between HB and PA ,TRF, RBP, RBC-C3bRR,and there is a negative correlation between HB and FDP . 6. N...
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