| Lung cancer is a kind of malignant tumor which greatly threaten the people's health and life. Recently its morbidity and mortality increase rapidly, and it has became the first tumor killer. It is critical for us to deal with the problems in the lung cancer's diagnosis and treatment. With the development of the medical science, especially the immunology and molecular biology, the studies of lung cancer have achieved greatly progresses. the findings and the appliances of VEGF(vascular endothelial growth factor), CD34 (cluster differentiation) and P16 gene are the signs of these progresses. Many researches have found that the occurrence, development and metastasis of lung cancer are some courses controlled by some genes, the tumor angiogenesis is the material and morphological foundation. Up to now, it is taken for granted that the tumor's growth undergoes a course of two stages, the one is proagiogenesis and the other is angiogenesis. In the proangiogenesis, because of the insufficiency of the vessel, it is hard for the tumor to sustain the size of few cubic millimeter, which is formed by the cluster of tumor cells. During this course, the tumor cell's proliferation (metabolism) was only by the means of simple passive diffusion. with the enlargement of the tumor size, Simple passive diffusion cannot afford the tumor cell's need of metabolism, meanwhile, the speed of the tumor's enlargement slows down. but it doesn't mean the speed of the tumor proliferation slow down. In the course of the neovascularization, The enlargement the tumr size speed up. Therefore, the ability of the invasion and metastasis also enhance. as a result, a series of the clinical symptoms emerge. The neovascularization is stimulated by some growth factors, such as VEGF, BFGF(Basic Fibroblast Growth Factor) and TGF(Transfer growth factor) secreted by the vascular endothelial cells. among these factors the VEGF takes the major function to stimulate the growth of the tumor and correlated with the tumor's proliferation, invasion and metastasis. VEGF was firstly found by the Ferrana and his coworkers at 1989. VEGF has the function to improve the activity of Endothelial cell's mitosis, it is found that there is a positive correlation between VEGF and MVD(microvascular density), therefore, VEGF has the function to improved the angiogenesis. There are many marks in the vascular endothelial cell, such as FVIIIRA,CD31,CD34 et al. At present the CD34 is reported that it has stability and sensitivity in the vascular endothelial cells, Especially in the capillary.P16 gene was found by the Serrano and his coworkers at 1993, it is also called as multiple tumor suppressor or CDK4 inhibitor. P16 protein coded by the P16 gene has the function that directly inhibits tumor's occurrence, as well as P16 protein can regulate and inhibit the cell's cycle.Out of the control of the cell's cycle results in the occurrence of the malignant tumor. Cancer gene and suppress cancer gene may indirectly and directly regulate cellular cycle. Their misfunction would lead to the happening of the tumor. Many literatures have verified that P16 gene feed back negatively to the normal cell cycle.Recently, VEGF,CD34 and P16 have been reported that they all have close correlation with tumor respectively, but it is rare to study the expression of VEGF,CD34 and P16 at same time in lung cancer, and to evaluate their clinical significance all over the world.In our study, streptaridin peroxidase (sp) immunohistochemistry for expression VEGF,CD34 and P16 gene were carried out in 82 cases of the lung cancer, the samples were collected from Ji Lin university 1, 2 and 3 hospital from 2001 to 2003, all the patients did not be treated with antitumor therapy before operation, we had the result.1. The expression of MVD,VEGF and P16 all had close correlation with the lymph node metastasis and the clinical stage (p<0.05).2. MVD,VEGF and P16 protein all had no relation with the patient's gender, age , histological types, histolog... |
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