The Correlation Study On The Expression Of DPC4 And VEGF In Cholangiocarcinoma

Objective:To study the expression of DPC4 and VEGF in Cholangiocarcinoma and their potential relationship with the clinical pathologic factors of Cholangiocarcinoma. Methods: The expression of DPC4 and VEGF in operative samples from 44 Cholangiocarcinoma patients were detected by immunohistochemistry. The vascular endothelial cells in tumor tissue were labeled by FVIII factor antibody for counting microvessel density (MVD). Results:1.The negative rates of the expression of DPC4 and the positive rates of the expression of VEGF in Cholangiocarcinoma were significantly higher than that in normal bile duct tissue(P<0. 05). In the lymph node metastatic group, the surroundings infiltrative group, the UICC III æ¡°V stage group and the middle and low segment Cholangiocarcinoma group, the negative rates of the expression of DPC4 and the positive rates of the expression of VEGF in Cholangiocarcinoma were significantly higher than those of the contrast group( P<0. 05). 2. In the lymph node metastatic group, the surroundings infiltrative group, the UICC IIIæ¡°V stage group and the middle and low differentiation grade group,MVD of Cholangiocarcinoma was higher than that in the contrast group (P<0.05). 3. In Cholangiocarcinoma, MVD in the negative group of DPC4 was significantly higher than that in the positive group of DPC4(P<0. 01).A negative correlation was presented between MVD and the expression of DPC4 (r= -0. 752, P<0. 001). At the same time, MVD in the positive group of VEGF was significantly higher than that in the negative group of VEGF(P<0. 001). A positive correlation was presented between MVD and the expression of VEGF(r= 0. 468, P=0.001).4.A negative correlation was presented between the expression of DPC4 and the expression of VEGF in Cholangiocarcinoma( r= - 0.49, P<0. 01). Conclusions: 1. The negative expressionof DPC4 and the positive expression of VEGF may play an important role in the occurrence and the development of the cholangiocarcinoma. 2. The negative expression of DPC4 may play a more important role in the occurrence and the development of the middle and low segment cholangiocarcinoma. 3. The decrease of expression of DPC4 may increase the expression of VEGF and the angiogenesis and facilitate the occurrence and the development of cholangiocarcinoma. 4. The negative expression of DPC4 and the positive expression of VEGF may promote the infiltration and the metastasis of cholangiocarcinoma. 5. The loss of expression DPC4 may be a late incidence in the carcinogenesis of cholangiocarcinoma.