| Objective: Pregnancy-induced hypertension syndrome(PIH), is a common and severe obstetric syndrome, and is a major cause of maternal and fetal morbidity and mortality, whose etiology and pathophysiology are still understanded. Recent data suggest that the immune imbalance has been implicated in the pathophysiology of PIH, with modification of the maternal cellular immunity. We observed peripheral blood and decidual immuno-associated cells and investigated the changes of the activities of induced cytokines, in order to know the correlation of the peripheral blood and decidual immunological factors, discuss the mechanisms of PIH and offer laboratorial evidences for preveneion and therapy of this disease. Methods: The study group consisted of 20 women with PIH, the control group consisted of 20 women with uncomplicated pregnancies. Women with the previous history of underlying hypertension, diabetes, chronic renal disease, platelet disorder, infection were excluded from the study. Two groups were matched for age and parity. Peripheral blood samples were obtained before antihypertensive treatment and labor. 10 milliliters of blood were taken by venepuncture in sterile conditions, and collected in sterile heparinized tubes. Maternal decidual tissues were obtained during elective caesarean sections and vaginal deliveries. Lymphocyte and monocyte/macrophage were isolated from peripheral blood and decidual tissue by centrifuge and enzyme digestion. Then lymphocyte was stimulated with ConA for 72h and calculated the ratio of lymphocyte transforming(LT). Lymphocyte and monocyte/macrophage were respectively cultured and stimulated with different kinds and concentrations of mitogens, then determined the activities of induced cytokines in the culture supernatants by enzyme-linked immunosorbent assay(ELISA). All data were analyzed with the SPSS 10.0 software, including t-test, ANOVA, linear regression and linear correlation.Results: 1 The changes of all kinds of decidual cells :Compared with control group, the number of large granulated lymphocyte(LGL) obviously increased in decidual tissue of patients with middle and severe PIH groups(P＜0.01). In mild PIH group, it was not higher (P＞0.05).2 The comparison of LT in peripheral blood or decidual tissue: Compared with control group, peripheral blood LT in patients with PIH was not higher(P＞0.05). Decidual tissue LT in mild PIH group was not higher than in control group(P＞0.05). But it was obviously higher in middle and severe PIH groups than control group(P＜0.01).3 The comparison of activities of induced cytokines in peripheral blood and decidual tissue lymphocyte: Compared with control group, the activities of IL-2, IFN-γin mild PIH group were not higher (P＞0.05), but in middle and severe PIH groups, they were obviously higher(P＜0.01). The activities of IL-4, IL-10 from peripheral blood lymphocyte decreased in patients with PIH. In mild PIH group, the activities of IL-4, IL-10 were not lower(P＞0.05), but in middle and severe PIH groups, they were obviously lower than control group(P＜0.01). Compared with control group, the activities of IL-2, IFN-γfrom decidual tissue lymphocyte in mild PIH group were not higher(P＞0.05), but in middle and severe PIH groups, they were obviously higher(P＜0.01). The levels of IL-4 from decidual tissue lymphocyte in PIH group were lower than control group, but had no significant statistical difference(P＞0.05). Compared with control group, The levels of IL-10 from decidual tissue lymphocyte in mild PIH group were not lower(P＞0.05),but in middle and severe PIH groups, it were obviously lower(P＜0.01).4 The comparison of the activities of induced cytokines in peripheral blood monocyte and decidual macrophage: Compared with control group, the activities of cytokines induced from peripheral blood monocyte and decidual macrophage, such as TNF-α, IL-6, were not higher in mild PIH group (P＞0.05), but in middle and severe PIH groups were obviously higher(P＜0.01). 5 The correlation of cytokines between peripheral blo...
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