| Objective:To investigate the effect of hepatocyte transplantation on acute liver failure (ALF) of rats. To investigate the change and significance of transforming growth factor-β1 mRNA ( TGF-β1 mRNA ) in liver after hepatocyte transpl- atation.Methods: 1.Preparation of ALF model. 10% D-gal solution was prepared and injected to rates via abdominal cavity with dosage of 1.6 g/kg respectively. 2.Cell seperation and purification. Collagenase perfusion was performed on six Wistar rats to separate liver cells and suspension was counted . 3.Hepatocyte transplantation experiments. Fifty-six of Wistar rats with ALF induced by D-gal were randomly divided into two groups: Group I and II. Group I was performed intrasplenic hepatocyte transplantation (approximately 2 ×107 cells ). Group II was performed intrasplenic injection of normal saline of same volume. 4.Post-transplantation liver function test. The blood samples of each group of experimental rates were drawn on the 6h , 12h , 24h and 48h after transplantation. ALT and TBil were assayed with automatic biochemical analyzer. Liver sample of rats was performed HE staining and in situ hybridization (ISH). Results: The comparison between group I and II shows that signficant differences were found in ALT and TBil of 24h and 48h( p< 0.01 ). Pathological changes of group I were much mild than its control group at 48h. The comparison between group I and II shows that no signficant differences were found in TGF-β1 mRNA of 6h and 12h(p<0.01). The comparison between group I and II shows that signficant differences were found in TGF-β1 mRNA of 24h and 48h(p<0.01). Conclusion: Intrasplenic transplantation could increase the index of hepatic function of rats with ALF induced by D-gal and hepatic pathology could be improved within 48 hours after transplantation. The decrease of TGF-β1 mRNA in gruop I maybe facilitate to hepatocyte growth. |
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