Expression Of CK20mRNA In Peripheral Blood Of Patients With Endometrial Carcinoma And Its Clinical Significance

Objective: to explore the role of peripheral blood CK20mRNA for the diagnosis of Endometrial Carcinoma. Materials and Methods: RT-PCR technique was used to detect CK20mRNA in peripheral blood of 40 patients with Endometrial Carcinoma before and after radical operation as well as 20 patients with benign endometrial disease.10 Endometrial Carcinoma sample and 20 peripheral blood from healthy women were used as positive and negative controls, respectively.Results: no expression of CK20mRNA were detected in peripheral blood in healthy women nor in patients with benign endometrial disease. Among the 40 patients with Endometrial Carcinoma, the positive rates of CK20mRNA in peripheral blood before and after radical operation was 27.5%(11/40) and 45%(18/40),respectively. The positive detection of CK20mRNA was significantly correlated with clinical stage; the higher the stage, the greater the positive rate. However, there was no significant correlation between the prevalence of CK20mRNA and pathological level or age of patient. The positive rate of CK20mRNA was higher after radical operation than that before radical operation. Conclusion: blood micrometastasis of the tumor can present even in an early stage of Endometrial Carcinoma.CK20mRNA is a good marker for detecting carcinoma cells in peripheral blood .The detection of CK20mRNA can be used to guide physician to judge clinical stage, to assess the prognosis and provide effetive treatment to patients with 9 Endometrial Carcinoma. Operation may precipitate tumor cell to spread into the blood.