The Relationship Between The Expression Of VEGF,P53, PCNA Protein And The Biological Behaviornesses Of Pituitary Adenomas

Pituitary adenomas is a high incidence intracranial tumors and accounts for about 10% of all intracranial tumor. Although most pituitary tumors are well differentiated,histologically benign neoplasms,their clinical behaviorness is known to vary greatly. Part of them have malignant tendencence , put up growth with invasiveness,showing that they spoil the bone surrounding them, and invade the dural, nerve, blood vessel. It is very difficult to be completely removed while presenting with surrounding important structure and internal carotid artery.The invasiveness of pituitary adenomas is a important reason of recurrence after operation, also is the reason of declineness of cure ratio. The invasiveness of pituitary adenomas have arouse many of the scholar to attach importance to it. Because the tumor cells of invasive and non-invasive pituitary adenomas have no distinct discrimination in models, it is difficult to distinguish the invasive pituitary adenomas from non-invassive pituitary adenomas by the means of the common tissue pathology, recognizing the mechism of pathogenesis and alteration of the biological behaviorness in pituitary tumors will help the improvement of diagnosis and treatment.We performed immunostaining assay using Streptavidin-Peroxidase (SP) mothed to detect the protein expressiones of vascular endothelial growth fact-or(VEGF), p53, proliferating cell nuclear antigen (PCNA) in 30 cases of inva-s-ive pituitary adenomas and 30 cases of noninvasive pituitary adenomas and to study the relationship between the expression of VEGF,p53,PCNA protein and the igenesis ,alteration of the biological behaviorness in pituitary tumors .The results shows that positive expression rate of VEGF, PCNA was 20/3 9(53.3%) , 17/30(43.3%) respectively in invasive group and 8/30 (26.7 %) , 5/30 (16.7%) respectively in noninvasive group . The postive expressions of VEGF, PCNA protein in invasive group were significantly higherthan that in noninvasive group(P<0.050). Positives staining of P53 protein was found in 7/39 (19.7%) of invasive pituitary adenomas and 0/30(0%) of noninvasive adenomas. Positive staining of p53 in invasive group was significantly higher than that in non-invasive group (P<0.010). Positive expression rate of VEGF, p53, PCNA in the first operation group were 6/9 (6 6.7%) , 2/9(22.2%) , 5/9 (55.5%) respectively, 6/9(66.7%) , 5/9(55.5 %) , 6/9(66.7%),in the second operation respectively, and 8/21(37.1%) , 0/21(0%) , 6/21(28.7%) in nonrecurrent group respectively. The recurrent group had a significantly higher postive expression of VEGF, PCNA protein than that of the nonrecurrent gro-up(P<0.05), and there no significantly difference between the first and second operation group(P>0.05). There was no significantly difference of the positive rate of p53 between the first operation group and the nonrecurre-nt group(P>0.05), however the positive rate of p53 in the second operation group was significant-ly higher than that of the recurrent group(P<0.01).Our expriment also shows that the expressions of VEGF have postive correlation with the expreesion of p53 protein , the expressions of VEGF have p ostive correlation with the expreesion of PCNA protein , and the expressions of p53 have postive correlation with the expreesion of PCNA. While playing r oles in pituitary adenomas respectively,they play roles cooperating with eath o ther.We conclude by the outcomes of our expriment that the abnormal expressions of VEGF, P53, PCNA protein may play important roles in the tumor ige-nsis and progression of pituitary adenomas and can be considered as the indicators of the invasiveness and recurrence in pituitary adenomas.