Isolation And Content Measure Of Tanshinone Compositions In Salvia Miltiorrhiza Bunge Var.alba

The paper is for the exploration and exploitation of a new source of tanshin, Salvia miltiorrhiza Bunge var. alba C. Y. Wu et H. W. Li, a variation of tanshin. The focus is on analyzing its chemical compositions and identifying relevant contents. Since new approach of composition isolation is applied, three compounds in tanshintone are isolated from Salvia miltiorrhiza Bunge var. alba C. Y. Wu et H. W. Li with high standard of purity. This provides key parameters for industrial manufacture. The content measure of tanshinone IIA from Salvia miltiorrhiza Bunge var. alba C. Y. Wu et H. W. Li and Salvia miltiorrhiza Bunge by HPLC proves that Salvia miltiorrhiza Bunge var. alba C. Y. Wu et H. W. Li is a substitute for tanshin to some extent. In the research, FR-980 BIAS is applied for the first time in record to measure the content of tanshinone IIA. Therefore, the field of chemical composition measure for Chinese herbal medicines has been enlarged.Tanshin, of the Salvia miltiorrhiza Bunge, is mainly found in Hubei, Shandong, and Henan Province. Since more and more wild plants gradually die out, it cannot meet demands after 1970s. 40 species of variation belonging to Salvia miltiorrhiza Bunge are being used as substitutes for tanshin. Salvia miltiorrhiza Bunge var. alba C. Y. Wu et H. W. Li, one of the variations, is only found in Shandong Province, with white or light yellow coronal, different from tanshin of purple coronal. In it, diterpenoid tanshinones with higher content than that of Salvia miltiorrhiza is always regarded effective in treating women diseases, decreasing blood liquid, lowering blood pressure and prolonging longevity. The wild species of Salvia miltiorrhiza and Salvia miltiorrhiza Bunge var. alba are so scarce that they were listed as among the rare and endangered plants to be preserved in 1987 by the State Council.In recent years scientific reports about Salvia miltiorrhiza can be frequently found, while the measure of Salvia miltiorrhiza Bunge's compositions and content is not people's attention. Up to now, only 14 compounds have been isolated from Salvia miltiorrhiza Bunge var. alba. Since 1990, systematic research has been conducted in Shandong Province for its taming, cultivation, quality criteria, effectiveness analysis, and toxicity tests. It proves thatSalvia miltiorrhiza Bunge var. alba is specially effective in vascular ectasis, with prosperous clinical use.In order to explore the untracked research field of Salvia miltiorrhiza Bunge var. alba, testify its medical value, and provide theoretical basis for quality appraisal, the paper makes a systematic research on three compounds by conducting novel isolation and identification approaches.The Salvia miltiorrhiza Bunge var. alba purchased from Tianyi Herbal Market in Laiyang City, Shandong Province was adapted as raw material in the research. In literature, all the approaches mentioned include alumina and silica gel column chromatography, and the poisonous solvents of benzene, ether, acetone, chloroform, and dichloromethane as well. The approach of Sephadex LH-20 column and ethanol or methanol as material is proved to be better than the above-mentioned methods and materials. My work focused on isolating three diterpenoid compounds by this means, with detailed flow as follows: take 1 kg dry tanshin root; ground and filter of 40 mm; then put into 10 L 95% ethanol for 24 hours a time, 3 repeats all together. After filtration, put the outcome into 100 % methanol, that is tanshinone. Put it to the Sephadex LH-20 column, with eluting liquid of 100% methanol, four-color sections (yellow, salmon pink, mauve, and brown-red) come into being. Filtered by silica gel plate, different color spots can be identified in the sunlight. Once more eluted by Sephadex LH-20 column, with eluting liquid of 50% and 70% methanol respectively, compounds of 92% purity can be extracted. The approach is easy to handle with low pollution and cost, meanwhile, the filling stuff in the column and the eluting liquid can be recycled. This approach is feasible for mass pr...