| Objective:Peripheral nerve autograft is the best method of repairing a long nerve defection .But the donar nerve is limited and resulting in certain function obstacle.Besides,it is necessary to solute that nerve growing speed is 1mm/d after nerve injury.In the near future,there are 2 metheds to solute nerve long defection ,one is muscle bridge,vein tube,silica gel of the tube, organize engineering and so on, but the research of these substituteses is placed in the stage of experiment.The other is nerve allograft to repair, but exsits the reaction problem of the immunity.Several methods of pretreatment of nerve allograft to lower its antigen contain chemistry method, refrigeration method, dry method, irradiation and so on. Another methods is the immunity depressant, prednisone, or applied at the same time all obtain the good result.It is one of the important factors that the speed is 1mm/d after nerve injury,it will be affect the curative effect.The muscle is out of contral after nerve injury long perid, the muscle fiber appears to atrophy, disvo- lution,fibrosis, losing the possibility that function instauration thoroughly.Therefore, It is aim for clinical nerve studying worker to accelerate the peripheral nerve regeneration speed from 1mm/d to 2-3mm/d. Peripheral nerve of the long defection and damage needs the two solution problems:one is how to bridge the nerve defection and damage, the other is how to accelerate nerve regeneration speed.But the new immunity depressant FK506 was found,it provides the technique supports, FK506 is separate from the streptomyces,it is a kind of antibiotics of macrolide. English name is tacrolimus, often translates for Te Ke Lei Mu or Te Ke Mu Si , his merchandise name is Pu Rui BO Si, FK506 assigns name for the laboratory. The main function of FK506(1) represses the rejection of organ transplants (2) accelerate the peripheral nerve regenration (3) nerve protection function.The clinical hand allograft found that FK506 can accelerate the regeneration peripheral nerve speed to 3 mm/ d.The experiment design(1) cold preservation of nerve allograft regeneration whether to surpass fresh allograft or not (2) cold preservation of nerve allograft regeneration with FK506 whether to surpass cold preservation of nerve allograft or not(3) the fit doses of FK506 of peripheral nerve regenration after cold preservation of nerve allograft .According to this we design this experiment.Come to investigate a method of allograft in an a kind of better proceeding nerve regeneration. Method:75 adult male winster mices,each weighing between 150 to 200g, is divided into 5 groups of 15 animals randomly,group A: fresh allograft,group B: cold preservation of sciatic nerve allograft,group C: cold preservation of nerve allograft received doses of FK506 (2 mg/ kg/ d),group D: cold preservation of sciatic nerve allograft received doses of FK506 (0.5 mg/ kg/ d),group E:isograft.Another 30 adult male SD mices ,serve as nerve donor,the two side siatic nerve were cut off and 15 of them were grafted to group A,45 of them were squently put into the glycerin for 3 hours each time whose concentration was 50%,70%,85%, then stored in the glycerin closeness bottle whose concentration was 85% and tempratrue was 5℃for 1 week,the nerve were put into the saline for 30 minutes before allograft.Each one all uses 11-0 nylon epineurial sutures was to repair the nerve defect,each animal received an allograft or an isograft .Each animal of group A,B,E received no medicine,group C received the doses of FK506(2mg/kg/d) for 8 weeks,group D received the dose of FK506(0.5mg/kg/d) for 8 weeks,the food and the oircunstance of the animals are the same. At 8,10 weeks postoperatively, the specimens were observed under naked eyes , light microscopy , the image analyzes of axons,the heavy of wet gastrocnemius,the limb sciatic nerve of the electrophysiological examination.At 8 weeks postoper- atively, the specimens were observed under electron microscope.To the E.M.G. result(MNCV,AMP DUR), the heavy of wet gastrocnemius...
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