Protective Effects Of Physcion On The Cerebral Injury From Ischemia-reperfusion

Cerebrovascular disease is a kind of common disease in clinic attacked by higher morbidity and unfavorable prognosis . It is seriously harm for the human health due to its first disability and lethality rate among three major causes of death ,the rate of cerebral ischemia is higher . Therefore , it is the one of the most important project of the clinical and experimental research to study pathophysiologic mechanisms and find the suitable treatment method for cerebral ischemia. It has been proved that thrombolytic therapy is first selected and effective measure for the patients with cerebral thromboembolism. However cerebral injury caused by ischemia-reperfusion(IR) would be more serious than ischemic injury itself . Ishchemic cerebral injury is a result of joint action of multiple phathophysiologic mechanisms with complicated procedure, in wihich the inflammatory response and apoptosis are very important processes. The rat model of focal cerebral ischemia was created by middle cerebral artery occlusion (MCAO) in the experiment. The level of on IL-1β by radioimmunoassay and the expression of ICAM-1 and Caspase-3 by immunohistochemical technology in the brain at the different reperfusion time (6h, 12h, 24h, 48h) together with effects of Physcion were observed. The research aimed to detect the effects of Physcion on inhibiting inflammatory cytokines and apoptosis in different reperfusion time after cerebral ischemia at early stage . The experimental results may be regared as the reliable basis of experiment and theory of clinic applying Physcion .1 The effect of Physcion on the level of IL-1β from cerebral ischemia-reperfusion in rats 　objective: To discuss the effect of physcion on the level of IL-1β from cerebral ischemia-reperfusion in rats　Meathods: The 105 healthy male SD adult rats were selected, and were randomly into normal group(Norm), sham-operated group(Sham) and cerebral ischemia-reperfusion group (Model) with 6 hours,12 hours,24 hours and 48 hours after ischemia reperfuson(IR), drug-control (NS) group ,low-dosage (20mg/kg) Physcion treating group (LD) and high-dosage (40mg/kg) Physcion treating group (HD) at 12 hours or 24 hours after ischemia-reperfusion. There were seven rats in every group. In the focal cerebral ischemia-reperfusion model , the right MCA was blocked up by a expanded-ended nylon filament for two hours ,and then the filament was pulled out . In the Sham group the nylon filament did not block up the MCA. The rats in drug treating group were administrated Physcion by stomach tube at 48h,24h before operation and just before operation. The rats in drug-control groups was administrated the same dosage's phydiological saline at the corresponding time point. At the end of the experiment the right part of the rats' brain was grinded into homogenate for detecting IL-1β by radioimmunoassay.　Results : The level of IL-1β at the different time point of Model group increased and not only had significant difference by one-way ANVON (F=293.049 P<0.01) but also were consistent in the time and quantity, and had significant difference compared with the corresponding time point of Sham group and Norm group(P<0.01). The level of IL-1β was at peak at 6h (1.295±0.0352ng/ml , compared with the Sham group P<0.01)after IR,then decreased gradually but reminded a higher level at 48h (0.9030±0.0212ng/ml, P<0.01) after IR. Compared with the corresponding time point of the NS group the level of IL-1β in Physcion treating group reduced and had significant difference (P<0.01).　Conclusion: IL-1β plays an important role in the cerebral ischemia-reperfusion injury. Physcion can inhibit the level of IL-1β from IR to protect the brain from ischemia-reperfusion injury2 The effect of Pyscion on the expression of ICAM-1 and Caspase-3 from cerebral ischemia-reperfusion in rats 　　Objective: To explore the effect of Pyscion on the expression of ICAM-1 and Caspase-3 from cerebral ischemia-reperfusion in rats 　　Methods: The 105 healthy male SD adult rats were select...