Studies On Radix Gentianae DNA Fingerprints And Quality Correlation

The purpose of this experiment was to build a suit of molecular biological and biochemical fingerprints methods about Radix Gentiana to meet quality standards of international.This experiment established the RAPD and ISSR fingerprints methods of Radix Gentiana through optimizeing RAPD,ISSR reaction system and other factors.Those two methods were applied in analysizing forty-three samples of four species Radix Gentianae.The total DNA templates used in this experiment were extracted by improved SDS method.Among these selected primers only 9 RAPD and 4 ISSR primers could use as genetic diversity analysis.The statistic data were analyzed to construct cluster analysis tree by using genetic distance UPGMA method.Analysis showed that genetic distances of G.rigeslens Franch to G.manshurica Kitag,G.trifiora Pall and G. scarbra Bge were far,and that of G.rigeslens Franch to G.manshurica Kitag,G.trifiora Pall and G. scarbra Bge were close.There were rich genetic diversity within every species.Estiblishing HPCE method at first to determinate gentiopicroside in Radix Gentianae.HPLC and HPCE were applied to analysize gentiopicroside contents in: Samples of same species ,same period and same site; Samples of same period and different species; Wild and cultivated samples of same period.Analysis of RAPD and ISSR fingerprints supplied molecular biological evidence for Radix Gentianain classification and growth. Quality analysis of HPLC and HPCE supplied scientific theories for Radix Gentiana in cultivation and collection.Candidate:CaoYa Nan Major: Preventative Veterinary ScienceSupervisor: Prof. Li Jingpeng...