Expression Of P～(27),PTEN,PCNA And TTF-1 In Benign And Malignant Thyroid Diseases

Objective: To research expression of P27 and PTEN﹑PCNA﹑TTF-1 protein in the different thyroid diseases.To investigate the role and clinical significance of P27﹑PTEN﹑PCNA and TTF-1 in the pathogenesis of thyroid carcinoma.Methods: The expression of P27﹑PTEN﹑PCNA and TTF-1 were examined immunohistochemically in 15 cases of nodular goiter,15 cases of adenoma and 43 cases of thyroid carcinoma.Results: Immunohistochemical staining results revealed that when compared with the P27 and PTEN positive rates of nodular goiter tissues(80.0% and 80.0%, respective) the P27 positive rates of thyroid carcinoma(34.9%) and the PTEN positive rates of thyroid carcinoma(46.5%) were significantly lower(P<0.01).In the nodular goiter tissues,the positive rates of expression was 26.7% for PCNA,and that in the thyroid neoplasma was 72.1%.The different expression of PCNA protein in nodular goiter and thyroid neoplasma was very obvious(P<0.01).The positive rates of expression of TTF-1 in nodular goiter tissues was 100%,and that in the thyroid carcinoma was 79.1%,the difference was insignificant between the two types.P27 expression was associated with histological type and infiltrative degree (P<0.05),but P27 expression wasn't associated with lymph node metastasis(P>0.05).The PTEN and PCNA expression were correlated with lymph node metastasis.(P<0.05).The expression of TTF-1 was not correlated with infiltrative degree and lymph node metastasis.There was significant positive correlation between the expression of P27 and PTEN.And negative correlation between P27 and PCNA.Conclusion: Loss of decreased expression P27 was significantly correlated with differentiation of thyroid carcinoma and infiltrative degree and markedly correlated with the expression of PTEN and PCNA.Combination of P27﹑PTEN and PCNA dection may be a powerful and aid in evaluating prognosis and infiltrative capability of thyroid carcinoma.TTF-1 was expressed in the majority of benign and malignant thyroid diseases.The immnostaining of TTF-1 was located in nuclear of cells,TTF-1 was a sensitive marker for thyroid follicular cells.