Correlation Between Protein Expression Of PTEN And VEGF, PCNA, Bcl-2 In Human Gastric Carcinoma

Objective: This study was designed to investigate the expression of PTEN and Vascular endothelial growth factor (VEGF),Proliferating cell nuclear antigen (PCNA), bcl-2 ingastric carcinoma and normal gastric mucosa , and the relationship between their expression and clinicalpathological factors.Methods: The expression of PTEN and VEGF, PCNA, bcl-2 in 68 cases of gastric carcinoma and 20 cases of normal gastric mucosa was detected using Immunohisto-chemical S-P method.Results: 33 cases were defected positive staining of PTEN(47.1%)in 68 cases gastric carcinoma, while all 20 cases normal mucosa showed positive staining of PTEN. The expression level of PTEN in high differentiated adenocarcinoma was higher than that in low differentiated adenocarcinoma. Also, the loss or decreased expression of PTEN was significantly correlated with lymph node metastasis and infiltrative deepness. In 68 cases gastric carcinoma, 51 cases (75.0%) and 50 cases(73.5%) were detected positive staining of VEGF and PCNA respectively, while in 20 cases normal gastric mucosa, only 2 cases and 4 cases showed positive staining of VEGF and PCNA, respectively, the expression of VEGF and PCNA were markedly correlated with infiltrative deepness and lymph node metastasis. 37 cases were defected positive staining of bcl-2 (54.4%)in 68 cases gastric carcinoma, while only 1 case in 20 cases normal mucosa showed positive staining of bcl-2 (5.0%).The expression of bcl-2 were not correlated with infiltrative deepness and lymph node metastasis.Conclusions: Loss of decreased expression PTEN was significantly correlated with differentiation of gastric carcinoma and lymph node metastasis and reversely correlated with theexpression of VEGF and PCNA. Combination of PTEN ,VEGF and PCNA detection may be helpful to evaluate prognosis and infiltrative capability of gastric carcinoma.