| Phosphatidyl inositol(PI) is widely present in cell membranes and endoplasmic reticulums. PI actively involved in the metabolism can produce many derivates by phosphorylation. As signal precursors, they can produce second messenger induced by protagonists. In addition, they have been implicated in many cellar events, such as ion transport, membrane trafficking, signal transduction, cytoskeletal organization and nuclear gene regulation. PI is used in food, health food, medicines, cosmetics, industrial preparation and so on, for its important physiological activities and characteristic emulsifying property.In order to make more exploitation on metabolism, physiological and medicinal properties of PI, it is very significant to extract and purify PI. Pure PI has been obtained up to now by meads of column chromatography, thin-layer chromatography (TLC) and high-pressure chromatography (HPLC) in our country. However, the yield thereof is very low. Soybean phospholipil is recognized as one of natural edible and medicinal phosphlipids. In this paper, we isolated and purified PI from Soybean phospholipil and the method has been applied to analyze the productions. Furthermore, the effects of PI on fatty liver in mice were also studied. The main conclusions are as follows:Making full use of the effects of polarity and PH of solvents on solubility of PI, the product which had a PI content of 96.5% was obtained through a limited number of simple steps: extracting with base ethanol; liquid-liquid distribution; precipitating with calcium chloride; employing diethylaminoethyl cellulose in which the PI was absorbed. A simple and efficient method for isolating and purifying PI in high purity was provided. Crude PI, refined PI and purified PI took turns to be isolated from purified soybean phospholipid power in 42.82, 7.71 and 0.35% yield, respectively.The content of PI was determined by thin-layer chromatographic scanner. Chloroform-ethanol-acetic acid-water (50 .' 25 I 10 : 2, by vol.) and Chloroform-ethanol-ammonium hydroxide (50 '. 25 : 4, by vol.) were used as developing solvent on a TLC silica plate made by ourselves. Developing distance was 12cm and bromothymol blue was used for coloration of the fractions. It could be concluded that the method is sample and feasible for the qualitative and quantitative analysis of PI.The animal model of fatty liver was reproduced using the multiple methods. Mice were fed with a high-fat diet consisting of 88% the standard diet, 10% lard and 2% cholesterol, and intraperitoneally injected a low dose of 0.1% (V/V) carbon tetrachlonde (CCU) at intervals to induce fatty liver model. To observe the effects of PI, mice were administered intragastrically PI for 10 consecutive days. We found that there was a trend toward a reduction in liver index. The cholesterol and free fatty acid in serum were decreased significantly. Furthermore, deteriorative lesions of hepatic tissue, such as fatty degeneration, infiltration were markedly improved. It is suggested that PI is effective in prevention and treatment of experimental fatty liver. The effect of antisteatosis is related to inositol that is the katabolite of PI by inference. |
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