| Objective: Primary hepatocellular carcinoma(PHCC) has become one of the most common malignant tumors in our country and has a high lever in malignancy and invasion. Intrahepatic metastasis often occurrences in early stage and the recurrence rate is high after operation. Studies indicate that there are two mechanisms of invasion and metastasis in hepatocarcinoma: one is tumor cells penetrate extracellular matrix(ECM) and vascular basement membrane and the other is angiogenesis. Currently, the investigation in tumor biological characteristic is more extensive and profound. Studies in heparanase(Hpa), basic fibroblast growth factor(b-FGF) and tumor microvessel density(MVD ) become the new hot spots. Heparanase is an endo-β-D-glucuronidase that destructs the integrity of ECM and vascular basement membrane and accelerates tumor cells invasion and metastasis. b-FGF is one of the fibroblast kindred and is one of the active angiogenesis factors. Recently, it is reported that b-FGF is closely related to the tumor angiogenesis. Combining the clinicopathological character of liver cancer, our experiment explore the relationship among the expression of Hpa, b-FGF protein , MVD and the occurrence, biological character of PHCC. We also explore their clinical values in predicting invasion and metastasis in PHCC.The study provides a theoretical or experimental foundation in tumor therapy, preventing recurrence and metastasis and evaluating prognosis. Methods: Forty-five tumor tissue samples and twenty -three tumor-surrounding tissue samples were obtained from PHCC patients undergoing surgical resection. Fourteen normal liver tissue samples were taken from previously benign tumor patients. Immunohistochemistry was used to detect the expression of Hpa, b-FGFand MVD. Statistical analysis was used to determine the relationship between their expression and clinical marker. Results: 1. Expression of the human Hpa, b-FGF protein: In PHCC twenty-six of 45 tumor tissues (57.8%) expressed Hpa and thirty-one(68.9%) expressed b-FGF. In contrast, only 6 of 23 tumor-surrounding tissues(26.1%) were Hpa positive and 8(34.8%) b-FGF positive. Two of 14 normal liver tissues (14.3%) expressed Hpa and six (42.9%)expressed b-FGF. Statistical analysis revealed that both Hpa and b-FGF expression were higher in tumor tissues than that in tumor-surrounding tissues and in normal liver tissues(P<0.05). 2. Numerical value of MVD is 28.17±11.54 in tumor tissue, 17.43±10.40 in tumor-surrounding tissue and 15.22±6.19 in normal liver tissue. Numerical value of MVD was significantly higher in tumor tissues than that in tumor-surrounding tissues and normal liver tissues(P<0.05).3. Relationship between expression of Hpa and b-FGF protein and MVD: Numerical value of MVD is 32.09±10.17 in Hpa positive tumor tissue and it is 31.09±11.22 in b-FGF positive tumor tissue. However, it is 22.81±11.37 in Hpa negative tumor tissue and 21.71±9.74 in b-FGF negative tumor tissue. Numerical value of MVD was higher in tumor tissues that Hpa and b-FGF were positive than that in negative tumor tissue(P<0.05). 4. Relationship between Hpa and b-FGF protein expression: Numerical value of MVD is 32.94±10.66 in both Hpa and b-FGF positive tumor tissue . However, it is 19.43±10.31 in both Hpa and b-FGF negative tumor tissue . Numerical value of MVD was higher in tumor tissues which both Hpa and b-FGF were positive than that in negative tumor tissue.There was a positive relationship between the expression of Hpa and b-FGF protein (P<0.05). 5. Relationship between the expression of Hpa and b-FGF protein and the clinicopathological character of hepatocarcinoma : The expression of Hpa and b-FGF protein has nothing to do with age, sex, the tumor size, hepatic function, AFP(P>0.05); but Hpa protein expression distinctly correlated with intrahepatic metastasis, lymph node metastasis, portal vein or biliary duct tumor thrombosis, the level of tumor differentiation(P<0.05); b-FGF protein expression associated with intrahepatic metastasis, lymph node metastasis, the level of tumor differentiation(P<0.05), but it was not related with portalvein or biliary duct tumor thrombosis(P>0.05). In patients with intrahepatic metastasis and lymph node metastasis inumerical value of MVD(32.57±12.64and34.98±11.22) is higher than patients without intrahepatic metastasis and lymph node metastasis(25.50±10.14and25.10±10.46) (P<0.05),but it did not relate to age, sex, the tumor size, hepatic function, AFP level, portal vein or biliary duct tumor thrombosis and the level of tumor differentiation(P>0.05). Conclusion: 1. Statistical analysis revealed that both Hpa and b-FGF expression were higher in tumor tissues than that in tumor-surrounding tissues and in normal liver tissues. Hpa and b-FGF were associated with the occurrence of hepatocarcinoma. MVD is one of the characters of hepatocarcinoma. 2. Numerical value of MVD was higher in tumor tissues that Hpa and b-FGF were positive than that in negative tumor tissue Suggest that Hpa and b-FGF can accelerate microvascular angiogenesis. 3. Numerical value of MVD was higher in tumor tissues which both Hpa and b-FGF were positive than that in negative tumor tissue.There was a positive relationship between the expression of Hpa and b-FGF protein. they are interauxo-action cooperative. 4. Hpa protein expression distinctly correlated with intrahepatic metastasis, lymph node metastasis, portal vein or biliary duct tumor thrombosis and the level of tumor differentiation,Hpa can regarded as a biological marker to...
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