| Acute respiratory distress syndrome(ARDS) is one kind of serious clinical syndrome caused by serious trauma, burn, infection and so on, whose characteristic is non-cardiogenic pulmonary edema, hypoxemia. The mortality rate of ARDS is over 50%. ARDS is regarded as one kind of general multi-internal organs damage. The death it caused is mostly because of the multi-internal organs function failure as well as complicating infection. The most important treatment of ARDS is machinery ventilates, which cannot reduce the mortality rate remarkably however. Therefore many scholars pay attention to medicine treatment and hope it can improve the cure rate of ARDS. But the clinical curative effect of medicine treatment is not remarkable. The peculiar medicine aimed at ARDS has not been found. At present the most significant treatment to ARDS is the whole internal organs' support treatment so as to protect every target organ. At the beginning of 2003 when severe acuterespiratory syndrome (SARS) occurred, Chinese medicine plays an important role in the battle of anti-SARS. It can obviously improve the SARS patients' symptom, reduce the probability of SARS patient to develop to the ARDS and prevent and control ARDS. Gardenia-Aweto Compound was used as clinical assistance drug in this anti-SARS battle and showed good clinical curative effect. But the mechanism of compound prescription to anti-ARDS is still not clear. The purpose of the research is to verify the effect of gardenia-aweto compound against ARDS induced by oleic acid in rabbit, and discuss its possible mechanism as well as the effective extraction method.Experiment 1 Objective To establish a rabbit model of ARDSinduced by infusion of oleic acid and to verify effect of Gardenia-AwetoCompound (GAC) in preventing ARDS by rabbits model induced byintravenous oleic acid. Methods Forty-eight New Zealand rabbits wererandomly divided into five groups, the blank control group, the modelgroup, and the GAC groups of high, middle and low dose. GAC wasadministered beginning from 3 day before modeling to the GAC groupsand to the other two groups, equal volume of saline was given instead.The ARDS model was established by intravenously injecting of oleic. Thedynamic changes of arterial blood gas, lung index (LI), wet/dry weightratio (W/D ratio) of lung, albumin content in bronchoalveolar lavage fluid(BALF) in different groups were observed and compared. Results Ascompared with the blank control group, in the model group, the arterialPO2 and SO2 deprived continuously, and the lung index, W/D ratio oflung, and content in BALF were significantly higher. While in the highdose GAC group, the above-mentioned parameters were all improvedafter treatment. But no significant difference was shown in comparisonbetween the parameters in the model group and the groups treated withlow or middle dose of GAC. Conclusion Intravenous injection of oleicacid is a reliable method for ARDS animal model. Preventiveadministration of high dose of GAC could alleviate the injury of oleicacid on lung in rabbits, to reduced the infiltrative pulmonary edema,elevate the blood oxygen saturation, so as shows a markedly effect inpreventing and treating ARDS.Experiment 2 Objective To study the effect of the two GAC components in preventing ARDS by model rabbits of ARDS induced by intravenous injection of oleic acid, and to detect the efficiency component of GAC in preventing ARDS. Methods GAC was divided into two components, ethanol-soluble components (ESC) and ethanol-deposition components (EDC) based on polarity. Forty-three New Zealand rabbits were randomly divided into five groups, the blank control group, the model group, the GAC group, the ESC group and the EDC group. The ARDS model was established by intravenous injection of oleic acid. Dynamic changes of arterial blood gas, LI, albumin content in BALF indifferent groups, lung histological were observed and compared. Results As compared with the blank control group, in the model group, GAC group, ESC grou...
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