The Effect Of Cinnabaris And Realgar In Angong Niuhuang Pulvis On HSP70,INOS And Cytokines

BACKGROUNDS: Angong niuhuang pill is the most famous medicine for emergency in Chinese traditional medicine .It is a fragrant drug for disspating heat, detoxifying, tranquilizing and resuscitation. It has a wide application in clinic practice on stricken by wind.The basis of cinnabaris is mercuric sulfide (HgS) . < Chinese pharmacopoeia > prescribe mercuric sulfide is not less than 96% .It has effect on tranquilization , hypnogenesis and therapy of epileptitrom convulsion .The basis of realgar is arsenic disulphide (AS2S2). < Chinese pharmacopoeia > prescribe arsenic disulphide is not less than 90% . It is antibacterial anticancer.OBJECTIVE: To study the effect of Angong niuhuang pulvis on HSP70,iNOS and CKs on pathological body and investigate initially the effect of cinnabaris and realgar therein providing scientific foundation for application in clinic practice.METHODS: Sprague-dawley rats including male and female were statistics randomly divided into two groups for 8 hours'and 24 hours' group . Both of them were divided in to 6 groups : normal control group , cerebral injury model group ,cerebral injury rats administrating cinnabaris , cerebral injury rats administrating realgar , cerebral injury rats administrating Angong niuhuang pulvis ,cerebral injury rats administrating Angong niuhuang pulvis excluding cinnabaris and realgar . At various times , the brain of rats were removed just after blood serum taken . The amount of HSP70mRNA ,iNOS in the rats' brain were estimated . So did the amount of CKs in the rat blood serum .RESULTS; The activities of HSP70mRNA of rat's brain in the groups administrating Angong niuhuang pulvis and Angong niuhuang pulvis excluding cinnabaris and realgar increase obviously compared with the cerebral injury model group 8 hours after injury (P<0.05) .The activity of HSP70mRNA in the group administrating Angong niuhuang pulvis is higher than that in the group administrating Angoug niuhuang pulvis excluding cinnabaris and realgar (P<0.05) .The activities of iNOS of rat's brain in the groups administrating cinnabaris, realgar, Angong niuhuang pulvis and Angong niuhuang pulvis excluding cinnabaris and realgar decrease obviously compared with the cerebral injury model group 8 hours after injury (P <0.05) .The activity of iNOS in the group administrating Angong niuhuang pulvis is lower than that in the groups administrating cinnabaris , realgar and Angong niuhuang pulvis excluding cinnabaris and realgar (P<0.05) .The activities of TNF- a of rat's serum in the groups administrating realgar, Angong niuhuang pulvis and Angong niuhuang pulvis excluding cinnabaris and realgar decrease obviously compared with the cerebral injury model group 8 hours after injury (P<0.05) . However, there were no significant difference among the activities of TNF- a in the groups administrating realgar , Angong niuhuang pulvis and Angong niuhuang pulvis excluding cinnabaris and realgar . The activities of IL-1 ^ of rat's serum in the groups administrating Angong niuhuang pulvis and Angong niuhuang pulvis excluding cinnabaris and realgar decrease obviously compared with the cerebral injury model group 8 hours after injury (P <0.05 ) .But the activities of IL-1 (3 in the serum have no significant difference between the the group of Angong niuhuang pulvis and that of Angong niuhuang pulvis excluding cinnabaris and realgar.The activities of serum and brain HSP70mRNA, iNOSJNF- a and IL-1 3 have no significant difference between the 24 hours' groups and the normal groups .CONCLUSION: The amount of HSP70mRNA is fewer in the normal rat's brain .It incresses obviously 8 hours after cerebral injury ; it comes back 24 hours after cerebral injury . The amount of iNOS is fewer fewer in the normal rat's brain .It incresses obviously 8 hours after cerebral injury ; it comes back 24 hours after cerebral injury . The amount of CKs is fewer fewer in the normal rat's brain .It incresses obviously 8 hours after cerebral injury ; it comes back 24 hours after cerebral injury.The activities of HSP70mRNA of rat's brain in the groups administrating Angong niuhuang pulvis and Angong niuhuang pulvis excluding cinnabaris and realgar increase obviously compared with the cerebral injury model group 8 hours after injury (P< 0.05) .The activity of in the group administrating Angong niuhuang pulvis is higher than that in the group administrating Angong niuhuang pulvis excluding cinnabaris and realgar (P < 0.05 ) . Neither cinnabaris nor realgar could induce HSP70mRNA by itself.