| Angong Niuhuang Wan,which is from Treatise on Differetiation and Treatment of Epidemic Febrile Diseases writtened by Wu Tang in the QIN Dynasty,is consist of Calculus Bovis,Cornu Rhinocorotics,Moschus,Margarita,Cinnabaris,Realgar,Rhizoma Coptidis,Radix Scutellariae,Fructus Gardeniae, Radix Curcumae,Borneolum Syntheticum. It has functions of clearing away heat and toxic material and eliminating phlegm for resuscitation,which can indicate high fever and delirium due to invasion of pericardium by pathogenic heat and retention of heat and phlegm within。Angong Niuhuang Wan can cure various acute cerebrovascular diseases with good effectiveness and light after-effect,which is the main chinese patent medicine of treating pyrexia with delirium and stroke. But because the components of Cinnabaris and Realgar include HgS and As2s2,the concentration of mercury and arsenic get above the standard,which is critical affect the clinical application and export of Angong Niuhuang Wan.Cerebrovascular disease is common disease of nervous system,which morbidity,mortality and disability is high. The important pathologial base of stroke is cerebral edema and hernia,which can cause a series of biochemical disturbance,such as energe equilibrium,electrolyte balance,enzyme system function,oxygen free radical and nerve transmitter content and function disturbance and so on. Tranditional Chinese Medicine take the view that the cause of stroke is deficiency of liver-yin and kidney-yin,and the symptoms of stroke is consist in wind,fire,phlegm and breathe,which can influence each other in the course of stroke. So Angong Niuhuang Wan can remove heat from the heart to resuscitate and protect brain tissues in order to awake the patients. Using the middle cerebral artery obstruction(MCAO)and bilarteral common carotid artery ligeature model,the present study was disigned to analyse the influence of Cinnabaris and Realgar in the Angong Niuhuang Wan's protective effects of brain tissues by comparing the effects of Angong Niuhuang Wan with un-including Cinnabaris and Realgar Angong Niuhuang Wan. The main results is as follow:1.Effects of the inflarct size of including and un-including Cinnabaris and Realgar Angong Niuhuang Wan in MCAO model ratsUsing FeCl3,we have made successfully MCAO model rats. The inflarct size of model rats is 4.51±2.25％,showing apparent cerebral ischemia injury. Angong Niuhuang Wan 3.0g/kg,1.5g/kg,0.75g/kg(coinciding with clinial dosage 25,12.5,6.3 times respectively)had obviously lessened the inflarct size in MCAO model rats,which shows Angong Niuhuang Wan can markedly improved cerebral ischemia injury. Cinnabaris and Realgar had not conspicuous influence in this effect.2.Effects of the brain water content of including and un-including Cinnabaris and Realgar Angong Niuhuang Wan in cerebral ischemia model ratsIn MCAO model rats,the brain water content of ischemia field was markedly increased,which had significant difference comparing with non-ischemia and control group. In cerebral ischemia-reinfusion model rats,the brain water content was obviously increased,which had significant difference comparing with control group. Angong Niuhuang Wan 3.0g/kg,1.5g/kg,0.75g/kg had the different degree in decreasing the brain water content of ischemia field in MCAO model rats；Angong Niuhuang Wan 2.4g/kg,0.8g/kg(coinciding with clinial dosage 19,6.3 times respectively) had obviously decreased the brain water content in cerebral ischemia-reinfusion model rats. The results shows that Angong Niuhuang Wan can protect brain tissues by protecting blood-brain barrier,decreasing capillary permeability,rising the brain tissues'stolerance to cerebral ischemia and reinfusion. Cinnabaris and Realgar had a little influence in this effect.3.Effects of the activity of SOD,CAT,GPX and the content of LPO,GSH of including and un-including Cinnabaris and Realgar Angong Niuhuang Wan in cerebral ischemia model ratsIn MCAO model rats,the activity of CAT,GPX was markedly drop... |
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