Protective Effect Of Matrine On Kidneys In Rats Of Glomerular Sclerosis

Objective:To evaluate the protective effect of Matrine on kidneys in rats of glomerular sclerosis and study its possible mechanisms . Methods:1.model and group:56 male Wister rats, randomly divided into control group A, n=8; control group B, n=8 ; model group A, n=10; model group B , n=10 ; treatment group A , n=10 ; treatment group B , n=10 . Control group A was normal control group, control group B was uninephrectomy group, Uninephrectomy and injecting by Daunoblastina (5 mg-kg)from tail vein to induced the rest rats glomerular sclerosis. Treatment group were injected Matrine (3mg·d-1, 1.5ml ) intraperitoneally . While other groups received only an equal volume of 0.9% saline 1.5 ml with the same schedule. After 4 weeks model group A , treatment group A were killed. 24 hour urinary protein, Scr, BUN and renal pathological chances were Assessed, calculated Glomerular Sclerosis Index(GSI), the expression of Col-Ⅳ and TGF-β1 were measured by immunohistological. After 8 weeks other groups were killed and each item was assessed as the same way.(1)control group A: normal control group + saline 8 weeks, n=8,control group B: uninephrectomy + saline 8 weeks, n=8; (2)model group A: uninephrectomy + Matrine + Daunoblastina 4 weeks, n=10, model group B : uninephrectomy + Matrine + Daunoblastina 8 weeks,n=10; (3)treatment group A: uninephrectomy + Matrine + Daunoblastina 8 weeks,n=10, treatment group B: uninephrectomy + Matrine + Daunoblastina 4 weeks, n=10. 2.index examination24 hour urinary protein, Scr, BUN , renal pathological chances and the expression of Col-Ⅳ and TGF-β1 measured by immunohistological. Results: 1.24 hour urinary protein , Scr and BUN(1)Compared with control group A. , each item of control group B was no significantly increased than control group A (P>0. 05).(2)Compared with control group , each item of each model or treatment group was significantly increased (P<0.01) ,(3)Between the same period treatment group and model group, each item was significantly decreased ( P< 0.01 or P <0.05) . 2 . renal pathological changes(1)PAS pigmentation: Between the same period treatment group and model group, treatment groups' renal pathological change are decreased than model group.(2)Glomerular Sclerosis Index: There were no glomerular sclerosis in control groups, between the same period treatment group and model group, treatment groups' are decreased than model group.3. expression of Col-IV(1) Compared with control group A. , control group B was no significantly change than control group A (P>0. 05) .(2) Compared with control group , expression of Col-IV of each model or treatment group was significantly increased ( P<0.01) .(3)Between the same period treatment group and model group, expression of Col-IV was significantly decreased ( P <0.05) .4. expression of TGF-1;(1) Compared with control group A. , control group 13 were no significantly changes than control group A (P>0. 05).(2) Compared with control group A, expression of TGF-Bi of each model or treatment group was significantly increased ( P<0.01 ) .(3) Between the same period treatment group and model group, expression of TGF-B1 of treatment groups were significantly decreased ( P <0.()5 ) . Conclusion:The renoprotective effect of Matrine in this moclle was confirmed by our observation of lower urine protein and reduced glomerular sclerosis Index , expression of collagen IV and TGI-1(1)Matrine can reduce 24 hour urinary protciiu Scr-. BUN.(2) Matrine can reduce renal pathological and GSI .(3)Matrine can reduce the expression of Col-IV and TGF-Bi. Matrine has protective effect on kidneys in rats of glomerular sclerosis. These effect maybe associated with reduction of ECM accumulation.