The Model Construction Of Partial Orthotopic Liver Transplantation With Hepatic Arterial Reconstruction In Rats And Its Significance

Background With liver transplantation being the effective therapeutic tool in treating the end stage and inborn hepatopathy, shortage of the liver graft is becoming one of the main reasons hindering the development of liver transplantation. Accordingly LDLT is developing quickly and obtaining more and more attention. As the improvement of liver transplantation technology, our research emphasis has turned to increasing the liver transplantation quality. In accordance with it, the establishment of consummate animal model of liver transplantation provides us research foundation.Objective: To develop the rat model of Partial orthotopic liver transplantation with hepatic arterial reconstruction, comparing with the rat model of classic two-cuff Partial orthotopic liver transplantation without hepatic arterial reconstruction and to comparatively study its significance.Metholds: 120 SD rats were randomly divided into 2 groups : Partial orthotopic liver transplantation group with hepatic arterial reconstruction(test group) and classic two-cuff partial orthotopic liver transplantation group without hepatic arterial reconstruction (control group). Changes in body weight, remnant liver weight, liver regeneration rate ,liver function, histological findings and proliferate index (PI) by flow cytometry were detected on the 1st, 2nd, 4th and 7th day after surgery respectively. Results: Alanine aminotransferase(ALT) and total bilirubin(TB) began to elevate from the first day after surgery. ALT in the control group on the fourth day and seventh day were significantly higher than those in the test group (P<0.05).TB in the control group at the fourth day were significantly higher than those in the test group (P<0.05).Histological findings indicated that more diploid and polyploid hepatocytes could be seen in the test group. PI in the test group on the second and fourth day were significantly higher than those in the control group [(32.81 ±3.45) %,(36.33±2.52)% vs (24.08 ± 4.66)%, (29.79±1.82%)]. Both remnant liver weight and liver regeneration rate in the test group were significantly greater on the fourth day. The proliferate index and Ki-67 labelling index of the graft in test group higher than that in control on the second and the fourth day after transplantation.Conclusions: (1) The modification of the technique provides a more practical and stable experimental model. (2) The reconstruction of hepatic arterial correspond to the physiological characteristics. (3)hepatic arterial reconstruction can significantly improve liver function of liver grafts afterPOLT, and promote the regeneration of liver grafts.