Study Of Growth Inhibitory Effects Of Static Magnetic Field On Cancer Cells

Aim: We studied the growth inhibitory effects of static magnetic fields (SMF) on cancer cells, and the synergistic anticancer effects between static magnetic fields and drugs.Methods: 1. the survival of human tumor cells (K562 cells and SMMC-7721 cells) and rodent hepatocytes treated by SMF were measured by MTT test. 2.The morphologic changes of K562 cells exposed under SMF were observed by microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM) and transmission electron microscopy (TEM); The cell cycle distribution and cytoplasmic calcium concentration ([Ca2+]i)of K562 cells after exposure were tested by flow cytometer (FCM) and ratio fluorescence measurement, respectively. 3. The influence of SMF combined with cyclophosphamide (CP) on K562 cells were detected by MTT test, TEM and FCM. 4. The kill effects of SMF cooperated with Chinese herbs on K562 cells were tested by MTT test.Results: 1.When cell density was 5×10~4cells/mL, the growth of K562 cells were inhibited after exposing under SMF for 12h (P<0.05) .While the cell density was 1 × 10~5 cells/mL ,the growth of K562 cells were supressed after exposing under SMF for 24h (P<0.05) . The proliferation of SMMC-7721 cells were declined after exposing under SMF for 9h (P<0.05) . There were no influence on growth of rodent hepatocytes which was treated under SMF for 24h (P>0.05) .2. After exposure, there were some changes in the morphology of K562 cells, such as some protuberances appeared on the cell membrane, mitochondria swelled, and nuclear membranes incrassated. When K562 cells were treated with SMF for 24h, the cell cycle distributions would changed, and ratio of cells at G1 and S phase was down, while those at G2/M phase was up. The cytoplasmic calcium concentrations of exposed K562 cells were lower than those of controls after 6h.3. When K562 cells were dealt with SMF and CP for 12h, the cell activities were significantly reduced (P<0.01), and the cell cycle distributions as well as ultrastructure were also changed, compared with controls.4. When K562 cells were treated with SMF cooperated with tubeimuside, chelerythrine or sanguinarine respectively for 24h, cell activities were significantly declined.