Bioeffects Of Moderate-intensity Static Magnetic Fields On The Growth Of Tumor Cells

Objective: This research is to investigate the bioeffects of exposure tomoderate-intensity static magnetic fields on the growth of mouse melanoma cells Bl6and human glioma cells U251, and provide a theoretical basis for the application ofSMF in the treatment of tumor disease.Methods: The three sets of experiments were conducted in this study.(1) AfterB16and U251cells were exposed to moderate-intensity static magnetic fields(50-200mT) for48hours and72hours, cell growth was measured by MTT assay toexamine the effect on cells viability;(2) Flow cytometry was used to detect cell cycledistribution in48hours of exposure to static magnetic fields, to explore the mechanismof the effects on tumor cell growth;(3) Biochemical methods were employed to monitorproteinase activity and antioxidant levels were relative to antioxidant defense system, todetermine the relationship between the effects on intracellular metabolic processes andthat on tumor cell growth.Results:(1) Short time exposure to SMF (50-200mT) could inhibit the growth ofB16and U251, but with increasing exposure time, the effects changed ingrowth-promoting;(2) There’s no effects on cell cycle distribution after exposure toSMF (100mT,200mT);(3) The GST activity and GSH/GSSG level displayed adownward trend after the first rise, while SOD activity and total antioxidant capacitydisplayed a rise trend after first decreased, and CAT activity showed no change afterexposure to SMF (100mT,200mT) for48hours.Conclusion: Moderate-intensity static magnetic fields could inhibit the growth ofB16and U251cells, and induced intracellular oxidative stress.