Expression And Functional Study Of SLP-2 Gene In Endometrial Cancer

Objective To characterize the differential expression of stomatin-like protein-2 (SLP-2) in endometrial cancer, and to study the potential role involved in carcinogenesis of human endometrial cancer.Methods (1) The expression of SLP-2 gene in 32 cases of endometrial cancer and 28 cases of normal endometrial tissues was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). And the SLP-2 expression was compared in endometrial cancer tissues with different clinicopathological data. (2) Eukaryotic expression vectors of sense and antisense SLP-2 were constructed and transfected into HEC-1B cell line using lipofectamine respectively. The morphological changes of the cell was observed by phase contrast microscopy. The cell growth was detected by methyl thiazolyl tetrazolium (MTT) assay, and the cell cycle were analyzed by flow cytometry.Results (1) The expression of SLP-2 mRNA in endometrial cancer tissues (1.58 ± 0.71) was higher than that in normal endometrial tissues (0.69±0.32) (P<0.05) . There was no correlation between up-regulated SLP-2 gene and clinical-surgical stage, myometrial depth, metastasis of lymph nodes, and differentiation (P >0.05). (2) Sense and antisense human SLP-2 constructs were transfected into HEC-1B cell line respectively. After being transfected with sense SLP-2, the expression of SLP-2 mRNA was increased by about 2.4 times more than that of the control group, the cell growth is accelerated, and the number of cells in G₁ phase was decreased by 12.5%, S phase was increased by 8.05%. After being transfected with antisense SLP-2, the expression of SLP-2 mRNA was declined 50%. The transfected cells showed slower growth by growth curve, and the number of cells in G₁ phase was significantly increased by 10.5%, and S phase was declined by 9.8%.Conclusions SLP-2 mRNA showed up-regulation in endometrial cancer tissues. It was suggested to have some relationship with regulation of cell cycle in endometrial cancer cell line, and was probably related to the carcinogenesis and development of endometrial cancer.