| Part one The Effect of the Lonicera Extract from Water Solution on the Growth of Bifidobacterium and Lactobacillus in vitro Objective To investigate the effects of Lonicera extract from watersolution (Lonicera ext) with various concentrations on Bifidobacterium andLactobacillus in vitro. Methods (1) Lonicera ext were extracted with pure water and dulited to 50%, 25%,12.5%, 6.25%,3.125%, 2%,1.0625% (wt/vol) with BL and MRS culture solution. At the same time, pure BL and MRS culture solution were used as the negative control. (2) Bif1101 and Lactobacillus bulgraticus were diluted to 6×108/ml after being relived, cultured under anaerobic conditions,washed with normal saline.(3) Bif1104, Lactobacillus were cultured for 20 hours in BL chopped-meat medium ,MRS medium with different concentrations of the Lonicera ext, then inculated the cultures to agar plates, counted colony-forming units(CFU) . (4) All cultures were inculated anaerobically for 24 hours, and in 0h,20h,24h, bacterium liquid culture were measured OD after being diluted 10 times and PH respectively, The E values were calculated to evaluate the stimulative growing effects of the Lonicera ext on Bifidobacterium and Lactobacillus. Results (1)The counts of Bif1101 and Lactobacillus in the solutionwith 1.0625%, 2%, 3.125% of the Lonicera ext were significantlyincreased. However, the counts of the solution with 12.5%, 25% , 50% ofthe Lonicera ext were markedly reduced. There were no differences in thecounts between the solution with 6.25% of the Lonicera ext and controlgroup. (2) The E values were higher than zero in Lactobacillus culturewith 1.0625%, 2%, 3.125% ,6.25% of the Lonicera ext, and the Evalues in Bif1104 culture with 1.0625%, 2%, 3.125% of the Loniceraext were higher than zero. (3) PH in Bif1104 and Lactobacillus culturewith 1.0625%, 2%, 3.125% of the Lonicera ext were significantlyincreased, and PH were no difference between the solution with 6.25% ofthe Lonicera ext and the control group. Conclusions The growth of Bifidobacterium and Lactobacilluswas promoted by the low concentration of Lonicera ext, and the highconcentration of Lonicera ext obviously inhibited the growth ofBifidobacterium and Lactobacillus in vitro. Part two The Effects of the Lonicera Extract from Water Solution on Intestinal Microflora of the Ovalbumin-Induced BALB/c Mice Objective To investigate the effects of the Lonicera ext onintestinal microflora of the ovalbulmin-induced BALB/c mice. Methods Female BALB/c mice aged at 4-5 weeks were raised on aovalbulmin-free rodent diet. The sensitized mice were randomly dividedinto group Y administered intragastrically the Lonicera ext after beingbasic sensitized , group T administered intragastrically the Lonicera extafter the first challenged with ovalbulmin (OVA) .At the same time, thesensitized mice treated with normal saline were assigned into group Ch asthe positive control . The normal mice fed with the Lonicera ext was alsoset as the control (group N). Meanwhile, the normal mice were set as thenegative control (group NS). Group Y, group T and group N were fed with100% of the Lonicera ext as group YH ,TH and NH , given 50% of theLonicera ext as group YM ,TM and NM , and treated with 25% of theLonicera ext as group YL , TL and NL.The levels of the OVA-specific IgEin serum of the mice were measured by ELISA. The tissues of smallintestine were histologically examined by either HE and toludine blue stain.The stool samples in cecum of mice were collected , diluted and culturedon specific selective agar plates to detect Bifidobacterium, Lactobacillus,Enterococcus , Staphylococcus and Enterobacteriaceae. Polymerase chainreaction and biochemical reactions methods were used to identifybacterium genus. Results (1) The levels of OVA-specific IgE in serum in group Y andT were reduced after being treated by gavage with 100% or 50% of theLoncera ext for 10 days.(2) The significant inflammatory reactions, such asthe accumulation of the lymphocytes and plasmacytes in lamina propriaand the focal necrosis an...
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