Intestinal Microflora Analysis For Type 1 Diabetic Murine

In recent years, the pathology of type 1 diabetes mellitus and its animal model is nearly well studied. The pathogenesis of diabetic murine model is thought to be determined by genetic defects, triggered by environment factors. Their close interaction results in the onset of type 1 diabetes mellitus. The former research on environmental factors mainly focuses on nutrient and virus infection, while little is touched on gut microecological system probably because of its inner complexity. Literature disclosed the disorder of intestinal microflora occurs in diabetic patients and animal models through isolation and culture methods. Our lab tries to study the possible relationship between diabetes and microbiota using molecular ecological methods. Some results indicate there is a certain connection between intestinal microflora and the onset and development of diabetes mellitus. Several bacterial strains were revealed directly associated with diabetes.In this study, ICR mice were randomly separated into two groups. One is for diabetes modeling, and the other is healthy control. After i.v. injection of STZ, diabetic mice were significantly lighter than control ones in weight, showing obvious diabetic symptoms. In addition, several model mice without hyperglycemia (STZ-resistant) were kept in comparison with diabetic ones.Firstly ERIC-PCR fingerprinting technique was used to profile the total DNA of fecal microflora samples and PLS-DA was used to search for the specialized features of different groups. Results indicated the gut flora of...