Human Papillomavirus (HPV) Testing In Diagnoseing Cervical Cancer And Cervical Precancerous Lesion

Human Papillomavirus (HPV) infection is one of the most common sexually transmitted infections in women.Many studies have indicated that HPV infections are the main cause of cervical cancers and cervical intraepithelial neoplasias (CIN) worldwide.The persistence of genital HPV infections is especially related to the development of cervical cancer. High-risk HPV genotypes have been detected in almost 100% of all cervical cancers.It would be useful to evaluate HPV testing to screen for cervical cancer. The second-generation Hybrid Capture 2 (HC II) test is a non-radioactive, relatively rapid, liquid hybridization assay designed to detect 13 high-risk HPV types(16,18,31,33,35,39,45,51,52,56,58,59,68).The HPV genotypes are related to cervical cancers and CIN,and divided women into high and low-risk groups. Objective:To investigate the relationship between the high -risk HPV and the cervical lesions,and the role of HPV testing in predicting CIN and cervical cancer. Methods:314 women who came to our gynecological clinic were evaluated for high-risk HPV infection with the HC II test. In the HC II assay ,a DNA specimen was denatured and hybridized with a full length RNA probes directed against a panel of 13 high-risk HPVs.The RNA-DNA hybrid were'captured'by hybrid-specific antibodies immobilized within 96-well plates,which was then detected by alkaline phosphatase-conjugated monoclonal antibodies to the RNA-DNA hybrid.The enzymatic activity was then detected by chemilumin escence and read by a microplate luminometer.Light measurements were expressed as relative light units(RLU).Cervical exfoliated cells were collected from the women aged 21-56 in a cross-sectional screening study.All women were subjected to cervical smears.HPV DNA was detected by hybrid capture 2 (HCâ…¡)system,and viral load was measured by the ratios of relative light units(RLU) compared to standard positive control (RLU/CO).Cervical lesions were diagnosed bycervical smears and biopsies as chronic cervicitis,CINâ… ,CINâ…¡-â…¢,and squamous cervical cancer. Results:Of the 314 patients 78 patients are infected by the high-risk HPV.The HPV-positive rate is 24.84%,and the HPV-positive rate in the 21-to 40-year-old patients is higher than in the 41-to 56-year-old patients,and the rate declines when age increases. The HPV infections rate is rising with the elevate of vervical lesions's level.The difference of infections rate between chronic cervicitis and CIN is significant(P<0.05),while is nonsignificant between CINâ… and CINâ…¡-â…¢(P>0.05).The median viral load for the positive women with chronic cervicitis,CINâ… ,CINâ…¡-â…¢and cancers are 33.86, 152.75, 208.14 and 598.30 respectively. The difference of viral load between chronic cervicitis and CIN is significant(P<0.05),while is nonsignificant between CINâ… and CINâ…¡-â…¢(P>0.05).The viral load ascents when the stage of cervical lesions is higher,which show that higher viral load could increase the risk of cervical lesions...