The Role Of The Occurrence And Metastasis And The Clinical Significance Of The Expression Osteopontin And Integrin α3,β1 Subunit In Colorectal Cancer

Objective To investigate the relationship between Osteopontin (OPN), integrinα3,β1 subunit expression and the occurrence and metastasis in colorectal cancer and the clinical significance. Methods S-P immunohistochemistry and situ hybridization, was used to determine the expression of OPN and mRNA to evaluate their effects on tumorigenesis and progression in 60 tissues of colorectal cancer: 32 of which had metastased to lymph nodes , 28 cases no metastasis. 12 cases adenoma, 13 cases normal mucosa. OPN expression was detected by Westernblot by the clinical fresh sample : 8 cases moderate differational colorectal carcinoma , 2 cases metastastic lymph node colorectal carcinoma and 1 case adenoma. S-P immunohistochemistry was used to determine the expression of integrin a3 andβ1 subunit in the same 60 samples. Results The rate of OPN expression in colorectal cancers was 80.0% (48/60), metastasis tumor was 84.3% (27/32), nonmetastasis tumor was 75.0% (21/28) ,adenoma was 91.6%(ll/12),but no expression was found in normal mucosa. OPN expression level had a correlation with the differentiation of tumor , lymph node metastasis and Dukes'stage (P <0.05) ;but had no correlation with the patients'...