Expression Of Osteopontin In Primary Bronchogenic Carcinoma And Its Clinical Significance

Objective To investigate the relationship between Osteopontin (OPN) expression and the occurrence and metastasis of lung cancer and its clinical significance. Methods The expression of OPN and its mRNA was determined by S-P immunohistochemistry and situ hybridization to evaluate their effects on tumorigenesis and progression in 57 cases with lung cancer tissues, of which 38 cases with metastasis of lymph nodes, and 30 cases with inflammatory pseudotumor and 20 cases with pulmornary bullae, which were used as control. At the same time, S-P immunohistochemistry was performed to analyze the expression of CD44v6 above the same samples. Results OPN protein and OPNmRNA were mainly stained in cytoplasm. CD44v6 was mainly stained in cytomeberance. The rate of OPN protein expression in lung cancers, inflammatory pseudotumor and bullae were 57.9%, 16.7%, 0% , respectively. The rate of OPNmRNA expression in lung cancers, inflammatory pseudotumor and bullae were 71.9%, 30, 0%, respectively. Statistic analysis showed that there was a significant difference between the lung cancer group and the pulmonary benign disease group (p<0.05). The rates of OPN protein and OPNmRNA expression in lung cancer tissues with metastasis were 71.1%, 86.8% respectively, the expression levels of OPN protein and mRNA were closely associated with lung cancer recurrence and metastasis respectively(p<0.05), and were not related to the sex, age, tumor grade, tumor size (P >0.05) . The rate of CD44v6 protein expression in lung cancers, inflammatory pseudotumor and bullae were 54.4%, 10.0%, 0 respectively. The rate of CD44v6 protein expression in lung cancer with metastasis were 65.8%. The expression of OPN protein was positively correlated to the expression of CD44v6(P<0.05). Conlusion Both OPN and CD44v6 may be involved in...