Studies On Chemical Constituents Of Leaves Of Piper Laetispicum C.DC

Piper laetispicum CDC, which only grows in China, belongs to the genus Piper, Piperaceae, widely distributed in Guangxi, Guangdong, Hainan provinces and some islands near the oceans. It is mainly used for injuries from falls, releasing ecchymosis and eliminating bump in folk medicines. Our group screened fifteen species of the genus Piper in South China, and found that the ethyl acetate fraction of the ethanol extract of Piper laetispicum C.DC roots and stems showed obvious activity against depression, moreover, found a new compound (named as laetispicine) had evident activity against depression. In addition, our group found the leaves contain more laetispicine than the roots and stems, and there is obvious difference between their HPLC spectrums, hereby we studied the chemical constituents of the leaves of Piper laetispicum C.DCSixteen compounds were separated and purified by the means of silica gel, ODS, Sephadex LH-20 and macroporous resin column chromatography from the ethanol extract of the leaves of Piper laetispicum C.DC, and identified their structures on the basis of physical and chemical methods and MS,NMR spectral methods.1: (2E, 4E)-N-Isobutyl-ll-phenylundecadienamide2: (2E, 4E)-N-Isobutyl-15-phenylpentadecadienamide3: Laetispicine4: (2E, 4E)-N-Isobutyl-7-(3', 4'-methylenedioxyphenyl) heptadienamide5: Brachystamide A6: Dihydropipercide7: (2E, 4ï¿¡)-JV-Isobutyleicosadienamide8: JV-p-Coumaroyltyramine9: rf-Sesamin10: Piperitol11: Vitexin12: Vitexin-2"-O-jS-D-glucoside13: (+)-Spathulenol14:j3-Sitosterol15: Stigmasterol16:1-DotriacontanolAmong them, compounds 1 and 2 are two new natural products, compounds 10, 11,12 and 16 are first isolated from the Piperaceae, and compounds 4,5,6,7 and 13 are obtained from Piper laetispicum CDC for the first time.