The Association Of TAP1 Gene Polymorphism And Hrpatitis B In Population Of North China

[Objective] : The aim of this study was to investigate the association between the single nucleotide polymorphisms (SNPs) of transporter associated with antigen processing gene1 (TAP1) and the susceptibility of hepatitis B in North Chinese.[Methods] : Genomic DNA of 176 hepatitis B patients and 208 healthy controls were extracted from the peripheral blood leukocytes. Then two fragments covering Codon333 and Codon637 of the TAPl gene were amplified by polymerase chain reaction (PCR). The SNPs of patients was revealed by restriction fragment length polymorphism (RFLP), the control by DNA sequencing. We use the software PHASE1.0 to construct the haplotypes of every individual. At last the unconditional Logistic regression model was used to analyze the statistical association of genotypes or haplotypes in two groups adjusted by gender and. age.[Results] : There are significant differences in the SNPs of Codon637 between the healthy control and hepatitis B patients in North Chinese: P<0.01 OR=6.34 (95%CI=2.49-16.13). Similarly, we find the combination of -/AG has a significant difference in two groups: P<0.01 OR= 19.85 (95%CI=8.33-47.31) .[Conclusion] : SNPs at Codon637 of the TAPl gene may relate to thesusceptibility of hepatitis B, and the risk of hepatitis B in participants with G/G allele is 6.34-fold compared to those with A/A allele. Furthermore compared with other haplotypes, ~/AG can rise the risk of hepatitis B 19.85-fold. These findings suggest that SNPs of TAP1 gene is one of the important host factors which independently influence the occurrence of hepatitis B.