The Pathological Changes And The Variety Of Dopaminergic Neuron Of Periventric Tissue In Experimental Hydrocephalus

Object Through the establishment of the hydrocephalus model of canine to evaluate the pathological changes and variety of the number of dopaminergic neuron of periventricle tissue.Methods A total of 14 adult male healthy mongrels were used for the experiment. 12 in laboratory group,2 in control group. Animals underwent baseline magnetic resonance imaging (MRI) and neurological evaluation prior to hydrocephalus induction. After the anaesthesia in vein and intubatton of branches , the canines were operated to exposure the Fourth ventricle through dura mater by posterior midmost way, and the cerebellomedullary cistern was exposured, then Cyanoacrylic glue was injected into the fourth ventricle of the laboratory group and the same amount of normal saline solution was injected in the fourth ventricle ofcontrol group through a canal, they all were monitored for 8 weeks post-induction using MRI and neurological fitness assessments. At the termination of the experiments, animals were deeply sedated and brains were extracted 0 The tissues of the brain, such as ependyma, white matter of periventricle, corpus callosum, hippocampus, were stained with hematoxylin and eosin (HE). At different degree of hydrocephalus, we can observe that many dogs of hydrocephalus can be find of symptoms like Parkinson's disease, such as hypokinesia, tonus, salivation, we use immuohistochemical method and use antibody link with tyrodine hydroxylase which is peculiarly in dopaminergic neuron and display dopaminergic neurons of basic ganglion through show the colour, we can ensure the changes of the mount of dopaminergic neurons in basic gaglion of hydrocephalus canines,so we can assure the pertinency of the symptoms and the the mount of dopaminergic neurons in basic gaglion.Results (1) In the laboratory group, one was died of anaesthesia, one was died of acute hydrocephalus, eight were successfully induced to hydrocephalus and assured by MRI, two of the twelve were found no difference. In the control group there were no difference after operation. (2)In the development of the hydrocephalus, we can review the abnormal behavior, such as inappetence, vomit, asthenia, lethargy, persistent gait and balance abnormity, rigor and hypokinesia.(3)In contrast with control group, the periventricular tissues of the experimental group developed thefollowing changes: widened fissures, flattened gyri, depressed grey and white matter, twisted hippocampi, prolonged fimbria hippocampi et al. The pathological changes were also viewed as the ependyma cells flattened like bug-corroded , the ependyma is discontinued with the structures completedly destroyed. The edema of priventricular tissues is mostly seen in the white matter. The number of the synapsis and other protuberances of the pyramid cells in the deep cortex of the control group is much less than the control group, the former is charactered by enlarged cell nucleus with darkened coloration, the perinuclear cytoplasm was less colored, and the astrocytes together with small glia cells developed reactive hyperplasia and hypertrophy. The endothelial cells swelled and the gaps between them were broadened. The nerve fibers of the callosum were also loosened and stretched.(4)We found that the amount of dopaminergic neuron declined compared to the control group in basic ganglion through immuohistochemical method and the larger of ventrical system, the less dopaminergic neuron could be found.Conclusion (D Canines can be used to establish the model of hydrocephalus by injecting Cyanoacrylic glue into its fourth ventricl. (2) We primarily observed and recorded the behavior changes of hydrocephalus canines, such as inappetence, vomit, asthenia, lethargy, persistent gait and balance abnormity, rigor and hypokinesia. (3) We primarily observe the widened fissures, flattened gyri, depressed grey andwhite matter, twisted hippocampi, prolonged fimbria hippocampi and the ependyma is discontinued with the structures completedly destroyed. The edema of priventricular tissues is mostly seen in the white matter. The number of the synapsis and other protuberances of the pyramid cells.? There is the pertinency between the symptoms and the decline of the number of dopaminergic neurons in the basic ganglion of hydrocephalus carmines,.