Experimental Study Of Its Mechanism Of Action Of Tetrandrine On Chronic Obstructive Pulmonary Disease Airway Remodeling

Objective: To study the effect of tetrandrine on rats with chronic obstructive pulmonary disease.Method:Fifty spragu-dawley rats were randomly divided into five groups:control group, COPD group,COPD+Qi deficiency syndrome+Tet group, COPD+Yin deficiency syndrome+Tet group, COPD+blood stasis syndrome +Tet group. The levels of TNF- α in BALF were measured with ELISA. The level of hydroxyproline in bronchial and lung homogenates was determined by biochemistry method. And the level of hydroxyproline in serum was also measured by biochemistry method. Lung tissue section stained by HE was observed to study the morphological alternations, inflammatory cells of the bronchial were counted. Thickness of the bronchial smooth muscle layer was measured by image analyzer. Expression and localization of TGF-β1 were observed in bronchi and lung tissues by immunohistochemistry. Result(1)The level of the COPD model group' s TNF-α (33. 92±8. 67pg/ml) , hydroxyproline in serum ( 13.82 ± 1. 15ug/ml ) and hydroxyproline in bronchial and lung homogenates (747. 3 ± 102. 9ug/g) were higher than the control group' s TNF-α (11. 12±5. 63pg/ml) , hydroxyproline in serum (7. 71 ± 1. 18ug/ml) and hydroxyproline in bronchial and lung homogenates (454. 0 ± 86. 5ug/g) , P<0. 01. The numbers of inflammatory cells in the COPD model group was significantly increased compared to the control group(P<0. 01). The COPD model group' s thickness of the bronchial smooth muscle layer (54.4 ± 7.8um) was significantly increased compared to the control group' s thickness of the bronchial smooth muscle layer(27.2 ± 1.8um),P<0. 01. The relative content of TGF-β1 in the epithelial cells of the bronchi in the COPD model group was significantly higher than that of control group(P<0. 01).