Effects On HBxAg Expressions Of Nude Mice Transplanted By Human Hepatic Carcinoma Cells And Significance Of HCC Prevention & Treatment Of Compound Phyllanthus Urinaria L.

Objective: To observe the effects on HBxAg expressions of nude mice (Balb/c-nu mice) transplanted by human hepatic carcinoma cells (HepG2.2.15 cells) and analyze the significance of Hepatocelluar carcinoma (HCC) prevention & treatment of CP (Compound phyllanthus urinaria L.). To explore whether the mechanism of the effects of CP on HCC is correlated the expressions of HBxAg (Hepatitis B x antigen).Methods: 40 Balb/c-nu mice were divided equably and randomly into five groups(1st, Prevention group with CP; 2nd ,Treatment group with CP; 3rd,Treatment group with CTX; 4th,Model control group; 5th,Blank control group) and supplied by different methods of administrations before or after making hypodermic tumor models in nude mice transplanted by HepG2.2.15 cells. To observe the tumor weight of different groups of nude mice, the content of peripheral serum AFP assayed by AxSYM system, and the expression of HBxAg in liver tissue and tumor tissue assayed by immunohistochemical methods.Results: The rate of success of nude mice transplant tumor models in the 1st group was 85.7%(6/7) which is 100%(8/8) in other model groups. And there's no statistic significance (P>0.05) between these groups. It existed an ubteraction of mice weight and observation periods among different groups (P<0.05). There was no significant difference of tumor weight between 1st, 2nd, and 3rd group (P>0.05). No significant differences of serum AFP concentration were observed between these groups (P>0.05). In experimental groups, the expression of HBxAg in tissue slices of livers were down regulated (P<0.05) as well as in tissue slices of Tumors. There was a comparability of the expression of HBxAg between tissue slices of livers and tumors: 4th>2nd>1st>3rd. But it was unclear about the relationship between the causes and the effects. In tumor tissues, the expression of the 3rd group was least, which of the 1st one took second place. There was no obvious difference between group l-3(.P>0.05).Conclusions: 1. HBxAg expressions can be observed both in tumor tissues and liver tissues of mice transplanted by human hepatic carcinoma cells. 2. CP can't stop the occurrence of transplanted tumor while can delay it and repress the growth of tumor. It could also inhibit the expression of HBxAg which is probably an important mechanism associated the efficacy of prevention & treatment of CP on HCC. 3. The relationship between CP and the growth of transplanted tumor can't be reflected clearly by the detection of AFP concentration in peripheral serum of nude mice. 4.Prophylaxis is probably the sticking point of prevention & treatment of HCC.