| ObjectiveTherapeutic approaches to periodontal regeneration in the past have utilized bone replacement grafts, growth factors, and barrier membranes. Recently Combined periodontal regenerative technique ( CPRT) has been used in treatment of periodontal defects. The effectiveness of CPRT has been evaluated in many studies in comparison to GTR, but conflicting results have been obtained. The aim of the present study was to evaluate the effectiveness of a combination of recom-binant human basic fibroblast growth factor ( rh - bFGF) - bovine - derived bone mineral collagen ( Bio - oss collagen) composite graft for periodontal regeneration in dogs.Materials and MethodsBilateral 3 - wall intrabony periodontal defects were surgically induced in the premolar region in the maxilla and mandible in 2 adult mongrel dogs. The defect in each animal received rh - bFGF - Bio - Oss collagen + Bio - Gide, or buffer — Bio — Oss collagen + Bio - Gide, and Bio — Gide. The animals were euthanized at 8week post surgery. Treatment outcomes were evaluated using clinical, radiographic, and histometric parameters (defect height DH; new bone formation NB; new cementum formation NC; new connective tissue adhesion CT).ResultsHealing was uneventful without infection. Eight weeks post - surgery, periodontal regeneration occurred in all sizes of the periodontal defect. Qualitatively new bone; cementum; connective tissue were observed. New bone formation in rh - bFGF — Bio - Oss collagen group(3.7 ± 0. 3mm) was significantly greater compared to buffer - Bio - Oss( 2.3 ±0.2mm ) and Bio - Gide( 1.2 ±0. lmm P <0.01). Respectly, new cementum formation averaged( ±SD) 2.3 ±0.2mm; 1.9 ±0. lmm; 1. 3 ±0.2mm,new connective tissue adhesion averaged( ±SD) 1.5 ±0. lmm;1.4 ±0. lmm;1.3 ±0.2mm, but the difference was not statistically significant in all groups( P >0.05 ). Histological analyses revealed that aberrant healing events such as root resorption and ankylosis were not observed.ConclusionsImplantation of rh — bFGF - Bio - Oss collagen may be used to support regeneration of alveolar bone in intrabony periodontal defects in dogs without aberrant events such as root resorption or ankylosis complicating the regenerative procedure. Rh - bFGF - Bio - Oss collagen does not have a significant effect on cementum regeneration and periodontal ligament formation in this study.
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