Apoptosis Induced By Artemether In SGC-7901 Cell Line In Vitro

Objective: To investigate the effect of artemether(ART) on the induced opoptosis in SGC-7901 cells and its mechanism in vitro. Methods:Inhibiting cell proliferation in different concentration and time was determined by improved MTT(Methy thiazolyl tetrazolium)assay. Alteration of morphology of ART induced apoptosis on SGC-7901 cells in vitro were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope. The fluorescence flow cytometry(FCM)DNA assay and TUNEL (Terminal deoxynuxleotidel transferase mediated uridine nucleotide and labeling) were applied to detect the changes of apoptotic rate at early and late apopotosis process and cell proliferation and alteration of cell cycle phase. Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation ratebecome slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis. Conclusion: ART possess induce SGC-7901cells apoptosis in vitro.