| Purpose 1.Search the methods of cleaning, selection of carriersand manufacture of the cutter. Aim at advantages and disadvantages ofseveral kinds of facture amnion ,to raise a normative method of factureamnion. 2.By observing and comparing the effects on curing rabbitcorneal alkali burn on using FAM,PAM, VXAM and VPAM, by observingthe ending of amnion on rabbit's ocular surface. Discuss the efficiency onthe different ways to keeping the amnion and find the functions ofbasement membrane and organism activity material. Method 1.To research the technics of preparing amnion.⑴Toobtain placenta from health pregnant woman at the time Caesareansection ,peel off the amnion, cleaning the surface of the amnion in twoways: saline and injector irrigating; saline irrigating and use carbasus toscrub the bloodstain on the amnion. Collect amnions in two treating waysto observe HE and TEM, to compare epithelia of amnion.⑵Put thecleaning amnions into 1000 U/ml cidomycin and 2.5μg /ml amphotericin B;2000 U/ml cidomycin and 2.5μg /ml amphotericin B for 20 min individual,4 hours later colture the amnions for bacteria, fungi and mycoplasm.⑶Tilethe amnion on operation paste scarf, 1um colloxylin coat, normal filterpaper and operating cap individual,observe the sticking stat in saline andglycerin preservation solution.⑷cutting amnion to patch by three kinds ofself-made trepans. observing and comparing the differences during wholeoperation. Then made them into FAM, PAM, VXAM and VPAM. 2. 60New Zealand rabbits were used to make corneal alkali burn models anddivided into FAM,PAM, VXAM, VPAM and CG, every group have 12rabbits. After operation, we observe the change of amnion transplant,corneal opacity, CNV and corneal fluorescein staining by slit lampmicroscope. 2 w, 1m,2m and 3m after operation, we did ultrastructuremorphological and histology observation. Results 1. On the technics of preparing amnion ⑴HE show that thedamage swashed by injector on amniotic epithelial are lighter than wipedby gauze. The former amniotic epithelial are close to integrity, the latter aredecoherence in some area. TEM show that there are damages on two kindsof amniotic epithelial.⑵The result of bacteria, fungi and mycoplasmcultivation are all negative. ⑶Colloxylin paper is the best choices onstickiness and physical capability; operation affix paper have goodstickiness . After cutting, it is easy to metamorphose. Filter paper can'tsupport the amnion after immerged in saline. It's fibre will fall off.Operating cap have good physical capability, but bad stickiness. It need tobe sutured with amnion.⑷All three kinds of trepans can cut amnion withcarrier effectively. The stamping trepan is the most convenient andlaborsaving, antisepsis is dependable. 2. Observe three months,FAMG,GPAMG and VXAMG have better curative effect than APAMG.APAMG and CG have not distinct difference. The amnion graft begin todissolve and desquamation after 18 days, as far as 1 month it disappearedcompletely. Amnion tissue didn't found under LM and EM. FAMG havebetter anti-CNV than GPAMG and VXAMG(P﹤0.05).FAMG,GPAMGand VXAMG have not distinct difference in corneal opacity andfluorescein staining(P﹥0.05). FAMG have the best curative effect.GPAMG and VXAMG have not distinct difference. Conclusions:1. On the technics of preparing amnion ⑴Because ofthe fragile of amniotic epithelia, it is easy to damage during gaining andcleaning, scrubing the amnions directely will damage the amniotic epithelia,so the action should be light and soft. Use the saline and injector flushingcan cleaning the bloodstain, protecting the epithelia, decreasing thedamages.⑵Lower concentration of 1000 U/ml cidomycin and 2.5μg /mlamphotericin B can assure effects of aseptic.⑶Colloxylin paper is the bestchoice as the carrier for it physical ability and stickiness.⑷The stampingtrepan can cut amnion expediently and normatively. It is easy to produce inlarge number. Simplify the operation.2.On transplantation ⑴ Aftertransplantation, VXAM,GPAM and FAM can accelerate corneal epithelialhealing, lighten inflam... |
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