| With the rapid development of hereditory and molecular andbiology , and the further research on oncogenes and anti-oncogenes , wehave primatively elucidated the mechanism of some kinds of tumor.The activation of oncogene and death of anti-oncogenes is themechanism of carcinoma development, which has become the hottestspot in biological reserch. OSCCs are the most common carcinomas inoral mucosa. Recently, some scientists tend to agree that its developmentand biological character is closely related with the change of some geneexpression. Pax9 is one the members of pax gene family, which isclosely related with the development and growth of oral andmaxillofacial structure, as well as their carcinoma. Some researchpointed that pax9 was expressed in normal epithelium of humanesophagus, while its expression decreased or even without anyexpression in transferred carcinoma. What's more, the ratio of pax9positive staining decreased with the increase of the extent of maligancy.This showed that pax9 could be used as accurate index of esophaguskerotinocyte differentiation ,and it also plays an important role in thedefferentiation of normal kerotinocyte. Pax9 maybe a anti-oncogene, asit is abundantly expressed in cells with karyokinesis, and mis-expressedin tumor cells. We want to find the related regulating mechanism ofOSCCs development by the expression pattern of pax9. We used the immunohistochemical staining to elucidate the pax9expression in normal and dysplasia of human oral mucosa and OSCCs,transferred OSCCs , trying to investigate the role of pax9 in developmentand mechanism of OSCCs.All the specimen came from Dept. of Oral Pathology, School ofStomatology, Jilin University, with 12 normal mucosa; 9 dysplasiamucosa and 45 oral squamous cell carcinoma, the latter were subdividedinto 24 Grade 1,15 Grade II and 6 Grade III . The 12 normal mucosawas taken as control. The slides were dewaxed, hydrated and culturedwith 3% H2O2. Then , they were cultured in normal goat serum and Firstantibody under 4°C overnight. The next day, they were cultured withSecond antibody and SABC under 37 °C . DAB was used as the colorreagent until the positive staining without background color appeared,then they were finished at re-staining. The known lung carcinoma specimen was taken as positive controland O.OIM PBS was added instead of First antibody to show the negativecontrol. The results were analysed under microscope as follows: A Score of staining: 0 was recorded when there was not any colorin the cells, 1 was recorded when it was slight yellow , while 2 wasbrown yellow and 3 was brown. B The ratio of positive carcinoma cells in all of them: 10 fields ofhigh magnificent were analysed for the number of positive cells. Theresults was shown as follows: 1 for 1-25% ; 2 for 26-50% and 3 formore than 75%. C The total score of every specimen= A*B? 0 score wasnegative ;l-3 score was(+) and 4-6 score was (++) and 7-9 score was.We used the SAS statistical software to treat the data and P<0.05 meantsignificant difference. In normal oral mucosa, pax9 was expressed in all the stratum ofepithelium and gradually strengthened from basal stratum towardscorneum stratum. In dysplasia epithelium with keratosis , pax9 wasmainly expressed in the cytoplasm of all the granulosum and spinosumstratum cells. While, in dysplasia epithelium without keratosis , pax9...
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