| Objective Human macrophage metalloelastase (HME), also referred as matrix metalloproteinase 12, has been shown to convert plasminogen into angiostatin, an essential inhibitor of tumor angiogenesis. The current study was designed to determine the role of HME in tumor angiogenesis, and to investigate the association of HME expression with disease progression in patients with gastric carcinoma.Methods Patients with gastric carcinoma who underwent surgical resection at Fist Affiliated Hospital of Anhui Medical University were followed up. HME expression in gastric carcinoma was analyzed by immunohistochemistry and hybridization in situ for archival paraffin-embedded tissue specimens in a comparative manner, and control groups was set up with chronic superficial gastritis and atypical hyperplasia. The relationship of HME, VEGF and MVD in gastric carcinoma tissue was analyzed, and the correlation between HME expression in gastric carcinoma and its clinicopathologic factors, along with the prognostic significance of HME, was evaluated. Meanwhile, frozen-stored fresh gastric carcinoma tissues were collected, and the difference of HME expression between tumorous (T) and adjacent nontumorous(N) tissues was analyzed by Western blot, and reverse transcriptase—polymerase chain reaction.Results Expression of HME was significantly higher in gastric carcinoma tissue than that in chronic superficial gastritis (P<0.01), and there is no difference of HME expression between carcinoma and over-medium atypical hyperplasia (P>0.05). In gastric carcinoma tissues, high-level expression of HME was significantly associated with hypovascular tumors (P<0.01). It is inverse correlation between expression of...
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