The Expression And Significance Of Microvessel Density Marked With Cd105 And Vascular Endothelial Growth Factor In Gastric Carcinoma

Objective:Study the expression of cd34 and cd105 in gastric carcinoma microvessel. To investigate the correlation between microvessel density (MVD) marked with cd105, expression of vascular endothelial cell growth factor(VEGF) and the invasion and metastasis in gastric carcinoma . Methods :VEGF expression and MVD in samples of 40 gastric carcinoma and 10 normal gastric tissue that collected in our hospital from 2003.03 to 2003.06 were examined by using immunohistochemical methods of S-P with anti-cd34 monoclonal antibody, anti-cd105 monoclonal antibody and anti-VEGF antibody respectively. Results:The mean value of MVD marked with cd34 and cd105 in gastric carcinoma were 47.18 + 17.93 and 20.38 + 8.31 respectively .In normal gastric tissue there were 9.30 + 3.34 and negtive expressing respectively. MVD and expression of VEGF was significantly higher in carcinoma withlymphnode involvement than that in normal gastric samples and in carcinoma without lymphnode involvement(p<0.01).There was a positive correlation (r=0.92) between MVD and VEGF. Conclution:The mean value of MVD marked with CD34 was higher than that marked with CD105(p<0.05). CD34 is more sensitive than cdl05 in counting MVD, but CD 105 is more specificity than CD34.MVD counted with CD34 or CD 105 is strongly related to the expression of VEGF and lymphnode metastasis in gastric carcinoma .Angiogenesis might promote the progression of gastric carcinoma and affect the prognosis of the patients.Assessing neovascularization with CD 105 in the process of gastric carcinoma development may be a valueble marker for predicting the risk of gastric carcinoma development.CD105 could be used in counting MVD as a new marker in angiogenesis.Both of the MVD and the VEGF could be used as the useful indexes reflecting the metastasis in gastric carcinoma.