| Objectives: The aim of the study was to explore the effect of estrogen-metabolizing genes and relative risk factors in the procession of the breast cancer. Thuswe can provide a foundation for clarifying the etiology of breast cancer, screening susceptible population, early diagnosis and effective prevention and treatment Methods: A hospital-based case-control study was conducted in Tianjin from July 2002 to December 2004. 100 normal women as a control and 105 women cases with breast cancer were recruited. The cases were considered to be breast cancer patients diagnosed by pathology and the controls were considered to be healthy individual with malignancy, procreation system disease, incretion disease, liver disease and kidney disease excluded. All the information was collected by questionnaire in face-to-face interview, which included demographic data, diseasehistory, disease family history, dietetic habit, behavior pattern, social psychologiccharacter, clinical data and laboratory data. The polymorphism of CYP1A1, GSTT1, GSTM1, COMT were assessed by polymerase chain reaction (PCR) and thePCR products were subjected to electrophoresis on agarose gel mat was stained with ethidium bromide. Unconditional logistic regression and ultivanate logistic regression were used to analysis the effect of the collected factors on the risk ofthe breast cancer.Results: Results from univariate unconditional logistic regression analysis showed that the following factors were significantly associated with the occurrence ofbreast cancer benign breast disease, first maternal age, dysmenorrheal, years of menstruation before primiparity, age of first term birth, times of pregnancy, duration of lactation, sterilizing operation, menopausal age, breast x-ray, BMI, intake of vegetables, meat, fried and fickle food, passive smoking, exercises andnegative affairs with OR(95%CI) 17.311(5.922~50.605),0.607(0.379~0.973),5.068(2.481~10.425),2.545(1.159~6.728),0.478(0.261~0.875),1.715(1.058~2.780),0.(H3(0.020~0.090),0.287 (0.134 ~0.612),2.787(1.477—5.260),1.543(1.020~2.593),0.583(0.374—0.908),2.000 (1.475 ~2.450),1.603(1.012—2.540),6.333(3.432—11.686),0.271(0.189—0.388), 0.604 (0.419~0.871),5.312(3.520~66.614) respectively. The results of multi-logistic analysis showed that the following factors were significantly associated with breast cancer history of breast disease (OR=12.292,95%CI:1.347~41.861), menopausal age(OR=3.6 24,95%CI: 1.474-16.907), passive smoking (OR=4.493,95%CI: 1.087— 18.571) and negative affairs (OR=5.842,95%CI:1.328~21.467) were risk factors of breast cancer. Sterilizing operation (OR=0.026,95%CI:0.002~0.332), duration of lactation (OR=0.299,95%CI:0.112—0.796) and outdoors exercises (OR=0.012, 95%CI:0.003—0.056) were protective factors of breast cancer. By regulating relative factors, analysis with respect to factors related to estrogen exposure among menstrual and reproductive factors indicated that die following factors were significantly associated with the occurrence of breast cancer Dysmenorrheal, years of menstruation before primiparity, pregnancy for many times were independent risk factors of breast cancer; duration of lactationwas protective factor of breast cancer. PCR-RLFP was applied to detect the polymorphism of CYP1A1,GSTT1, GSTMland COMT. The result shows that significant difference was found among the distribution of GSTTl, GSTMl and COMT between controls and cases. There was a positive correlation between CYP1 Al, COMT and breast cancer wife the OR of 1.663 and 1.548 respectively. Therewas a negative correlation between CSTT1, GSTMl and the risk of breast cancer with OR of 2.133,2.458 respectively. Polygenic model analysis indicates: CYP1A1,GSTT1,GSTM1 were independent risk factors of breast cancer, besides, there was a statistically significant correlation between the interaction of GSTT land GSTMl and the incidence of breast cancer which revealed that individual in simultaneous lack of GSTTl and GSTMl would run 10 times as high risk asnonnal persons do...
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