Variety And Quality Study On Radix Curcumae

Radix Cureumae, the root tuber of Curcuma longa L. , C. wenyujin Y. H. Chen et C. Ling and C. kwangsiensis S. G Lee et C. F. Liang or C. phaeocaulis Val, root in the family of Zingiberaceae. The classification , identification and quality control of Radix Cureumae materials are difficult as the result of their complex origin, wide distribution, multi-variety and fruits with blooms of most species hard to pick. The work in this thesis is part of the Tackling-key Project of Sichuan Department of Science and Technology : Investigation on GAP of Radix Curcuma. We study the variety and quality of 9 samples on plant morphology, material character, micro-character, molecular biology, chemistry ingredient and pharmacological effect.From the study on morphological and histological, We find the identifying characteristics on plant morphology, material character and micro-character of different varieties, we described the detailed transection structure and powder micrograph of huangsi yujin and the brief transection structure of six ther samples.We first time use SRAP technology to study 9 samples on molecular biology. By selecting of primer and optimizing the process we get better amplification effect. Each primer pair could develop 14.7 polymorphism b and 25 at the best, which proved SRAP technology applying on the molecular biology of Radix Cureumae. Through the study of plant morphology, medicinal material characters, micro-character and chemistry ingredient analysis we divide 6 varieties into 2 groups : one is huangsi yujin and huangbaisi yujin and the other is lvsi yujin, wen yujin ,gui yujin and baisi yujin. We Consider huangbaisi yujin as a cultivar of C.longa L., nameed C.longa L.cv.chuanyujin and baisi yujin as a cultivar of C. phaeocaulis Val. ,named C. phaeocaulis Val. cv. baisi.Through the TLC identification of carbinol extraction and essential oil, the check of moisture and ash, the assaying of essential oil, curcumioids,curcumin and extracturm ,we find the component and content of different varieties are obviously different, while the difference between the different sourced sample from a same variety is less. The content of essential oil, curcumioids and curcumin of huangsi yujin is the highest:essential oi can get 2.5%, cucumin can get 0.12 % and curcumioids can get 0.309%.We research on the pharmacological effect of YuJin on reducing blood fat, blood rheology and anticoagulation. The results show Huangsi yujin, huangbaisi yujin, lvsi yujin and wen yujin have better effect and baisi yujin and gui yujin have no effect. The carbinol extraction of Radix Cureumae is the efficient section. We suggest that baisi yujin and gui yujin should no longer be used in medicine as Radix Cureumae and lvsi yujin and wen yujin should be used separately. Curcumin is the available component of huangsi yujin and huangbaisi yujin, which is the control index.The results provide the scientific theory basis for the variety identification, quality estimate, the establishment of available component, the set up of quality standard and the foundation of Radix Cureumae GAP base.