Taper Leaf Of Bupleurum Chemical Composition And Quality Research

Radix Burpleuri is commonly used bulk herbs with a long history. And have the effect of reconciliation table, dewatering, sun's functions, hepatoprotective, antipyretic and anti-tumor. What contained in Chinese Pharmacopoeia 2010 Edition was the dried root of Bupleurum Chinese DC. and Bupleurum scorzonerifolium Willd. According to their different characteristics, they were called "North Bupleurum" and "South Bupleurum" according to their different characteristics. There are many species and widespread, and Radix Burpleuri in the place of origin are for medicinal use, causing the species problem. This paper studied the non-pharmacopoeia" Hailer" Bupleurum form Inner Mongolia appeared in Hebei Qizhou drug transactions, and identified it belong to Bupleurum bicaule Helm. There are large quantities of "Hailer" Bupleurum herbal to sell for medicinal use in three trees medicine market, Harbin.PartⅠLiterature Review:accessed and summarized the research data of the Bupleurum, Bupleurum and Bupleurum cone leaves a variety of medicinal plants, literature review from the aspects of the original plant morphology, drug source investigation, identification, quality, chemical composition and pharmacological. A Bupleurum information database was built with Microsoft Access software to facilitate information management and query. Just a little about radix Burpleuri Bicauli, but the shape and characteristics of plants.PartⅡthe experimental results:Ⅱ-A collected and identified the Hailar Bupleurum form Inner Mongolia Autonomous Region (radix Burpleuri Bicauli), according to microscopic characteristics of plant morphology and fruit, the characteristics and microscopic characteristics of medicinal parts of the root, determined the Hailar Bupleurum original plant leaf cone is Bupleurum bicaule Helm.Ⅱ-B studied the root of Inner Mongolia radix Burpleuri Bicauli and Qizhou drug market "Hailer" Bupleurum through the methods of medicinal character identification, microscopic, and the chemical identification, and Identified Hailar Bupleurum in Qizhou medicine market is the dried root of Bupleurum bicaule Helm.Ⅱ-C the chemical constituents of radix Burpleuri Bicauli1. it is the first time to analyze cone leaves Bupleurum volatile oil components, and it is found the similar with components of Bupleurum volatile oil. However, it is significant different from North Bupleurum, the two have no common components. The first three components are 1,2,3,6,7,8,8 a,8b-8 Hydrogen 4,5-Dimethyl-biphenyl support 17.43%,11-oxa-tetracyclic [5.3.2.0 (2,7).0 (2,8)] dodecyl-9-one 12.68% and (R)-1-methyl-4-(1,2,2-trimethyl-cyclopentyl)-benzene 10.36%. 2. The chemical constituents of radix Burpleuri Bicauli were isolated and identified, the ethanol extract with AB-8 macroporous resin enrichment, silica gel column chromatography was eight compounds.Using spectral analysis, chemical analysis to identify six compounds structure, namely:saikogenin F, prosaikosaponin G, Saikosaponin d, saikosaponin a, saikosaponin b2, Saikosaponin c. Is the first time be obtained from the radix Burpleuri Bicauli.Ⅱ-D Determination1. Determination the main saponins of radix Burpleuri Bicauli determinate the Bupleurum saikosaponin a, c, d content by High performance liquid chromatography. Three batches of radix Burpleuri Bicauli's saikosaponin a, d, c content are respectively,6.64～7.25 (mg/g),14.61～15.71 (mg/g),2.76～3.20 (mg/g). The average content was 7.12 (mg/g),15.17 (mg/g),3.00 (mg/g). Three batches of Bupleurum average content of total saponins is 25.23 (mg/g). Determination of the average concentration of the two groups of North Bupleurum saikosaponin a, d, c is 10.77 (mg/g),12.55 (mg/g),2.08 (mg/g). Two groups of North Bupleurum saponin is 25.40 (mg/g). Comparison of two Bupleurum found that radix Burpleuri Bicauli's Saikosaponin d is on higher levels, little difference between the total saponin content.2. Determination of volatile oilAppendix of the essential oil using Pharmacopoeia assay method, determined radix Burpleuri Bicauli volatile oil content, oil yield rate was 0.19%.Ⅱ-E Preliminary study of cone leaves of Bupleurum fingerprintBy high performance liquid chromatography, a preliminary study of the chemical constituents of radix Burpleuri Bicauli fingerprints was conducted. Identified 27 peaks.Ⅱ-F drafted the radix Burpleuri Bicauli quality standards; provide a theoretical basis for its clinical application.These studies have shown that the main chemical components of radix Burpleuri Bicauli are similar with the north Bupleurum included in the 2010 version of pharmacopoeia, can be medicinal use to relieve the shortage of resources of wild Bupleurum, with the development and application prospects value. However, significant differences in volatile oil, and whether having the same pharmacological efficacy need to be further studied.PartⅢinnovation in this paper1. it is the first time to identify the "Hailer" Bupleurum on Qizhou drug market is the dried root of Bupleurum bicaule Helm.2. it is the first time to conduct a reach on the radix Burpleuri Bicauli volatile oil and chemical composition of saponins, isolated and identified 7 saponins.3. it is the first time to formulate the radix Burpleuri Bicauli quality standards, provide a theoretical basis for its application.