| Objective To study the relationship between the pathological procedure of pulmonary candidiasis albicans generation and development and serum enolase ,to provid theory basis for serological diagnosis of pulmonary candidiasis albicans.Methods We adopt on the method of per cutem puncturation airtube to establish the animal model of pulmonary candidasis albicans ,we choice 30 male New Zealand White rabbits weighting 2 to 2.5 kg ,devide them into two groups randomly, experiment and control animals are all 15.In every group ,We devide into five subset groups , every subset groups animals are all 3. First, we use cytarabine intravenous injection to set up the animal model of immune suppression. At the same time, intravenousinjection antibiotics to prevent the bacterial infection. Second, we use the method of per cutem puncturation airtube to intratracheal injection bacterium fluid 0.2ml involvement 1×10 Candida albicans, form the animal model of pulmonary candidasis albicans. The control group intratracheal injection aequale normal sodium as comparison. We kill the animals in 1,2, 3, 4, 5 day after infection according to the sequence of A, B, C, D, E groups and get the blood, abstract the animals lung, fixture the lung in 10% formalin more than 24 hours. Then, we do pathological section and carry out PAS drum dyeing, observe the lung tissue pathological change. The management of animal serum, we detect the absorbance (OD optimum)at different concentration gradient of standard antigen and standard antibody by mean of euzymelinked immunosorbent assay (ELISA) and search the optimal antigen and antibody concentration. Then, draw the standard curve of antigen to be used to detect. After that, we detect the serum enolase absorbance in the same condition at the different period after the animals are infected. We obtain the serum antigen concentration at different periods. By the standard curve, we make the dependability between serum antigen concentration and pathological change, to explore the internal connection both of them. By the standard curve, we may know the serum antigen concentration when histology just emerge pathological change. We may think the serum antigen concentration as the earlier serological diagnosis evidence ofpulmonary candidasis albicans, provid earlier and rapid diagnosisevidence.Result 1. There are macroscopic focus of infection in postvaccinalanimals lung, may touch noduses on it. There are sporuses and hyphas inthe lung tissue under the microscope. There are suppuration and necroticfocus of injection, and emerge typical pathological change of pulmonarycandidiasis albicans.2. The best antigen concentration used to detect serum enolase antigen by ELISA is 0.4 μg/ml.3. The serum enolase concentration gradually step up with pathological change aggravation. It take on positive correlation between animals infection time.Conclusion 1.The methods of per cutem puncturation airtube may successfully set up the animal model of pulmonary candidiasis albicans. The operation of the method is convenient. The reproducibility is better.2. Between serum enolase antigen content of infected animals and pathological change of pulmonary candidasis albicans take on positive correlation, serum enolase may early diagnosis pulmonary candidasis albicans.3 .The earlier period of rabbit pulmonary candidasis albicans, the serum enolase concentration is 3.8 μg/ml. |
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